TY - JOUR
T1 - Cationic state distribution over the chlorophyll d-containing P D1/PD2 pair in photosystem II
AU - Saito, Keisuke
AU - Shen, Jian Ren
AU - Ishikita, Hiroshi
N1 - Funding Information:
This research was supported by the JST PRESTO program (H.I), Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan ( 21770163 to H.I. and 22740276 to K.S.), Special Coordination Fund (H.I.) for Promoting Science and Technology of MEXT, Takeda Science Foundation (H.I.), Kyoto University Step-up Grant-in-Aid for young scientists (H. I.), and Grant for Basic Science Research Projects from The Sumitomo Foundation (H. I.).
PY - 2012/8
Y1 - 2012/8
N2 - Most of the chlorophyll (Chl) cofactors in photosystem II (PSII) from Acaryochloris marina are Chld, although a few Chla molecules are also present. To evaluate the possibility that Chla may participate in the P D1/PD2 Chl pair in PSII from A. marina, the P D1•+/PD2•+ charge ratio was investigated using the PSII crystal structure analyzed at 1.9-Å resolution, while considering all possibilities for the Chld-containing P D1/PD2 pair, i.e., Chld/Chld, Chla/Chld, and Chld/Chla pairs. Chld/Chld and Chla/Chld pairs resulted in a large PD1 •+ population relative to PD2•+, as identified in Chla/Chla homodimer pairs in PSII from other species, e.g., Thermosynechococcus elongatus PSII. However, the Chld/Chla pair possessed a PD1•+/PD2•+ ratio of approximately 50/50, which is in contrast to previous spectroscopic studies on A. marina PSII. The present results strongly exclude the possibility that the Chld/Chla pair serves as PD1/PD2 in A. marina PSII. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
AB - Most of the chlorophyll (Chl) cofactors in photosystem II (PSII) from Acaryochloris marina are Chld, although a few Chla molecules are also present. To evaluate the possibility that Chla may participate in the P D1/PD2 Chl pair in PSII from A. marina, the P D1•+/PD2•+ charge ratio was investigated using the PSII crystal structure analyzed at 1.9-Å resolution, while considering all possibilities for the Chld-containing P D1/PD2 pair, i.e., Chld/Chld, Chla/Chld, and Chld/Chla pairs. Chld/Chld and Chla/Chld pairs resulted in a large PD1 •+ population relative to PD2•+, as identified in Chla/Chla homodimer pairs in PSII from other species, e.g., Thermosynechococcus elongatus PSII. However, the Chld/Chla pair possessed a PD1•+/PD2•+ ratio of approximately 50/50, which is in contrast to previous spectroscopic studies on A. marina PSII. The present results strongly exclude the possibility that the Chld/Chla pair serves as PD1/PD2 in A. marina PSII. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
KW - Acaryochloris marina
KW - Chlorophyll d
KW - P680
KW - Photosystem II
KW - Redox potential
KW - Spin density distribution
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U2 - 10.1016/j.bbabio.2011.12.003
DO - 10.1016/j.bbabio.2011.12.003
M3 - Article
C2 - 22192718
AN - SCOPUS:84862239819
SN - 0005-2728
VL - 1817
SP - 1191
EP - 1195
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 8
ER -