Cationic liposome can interfere mRNA translation in an E. coli cell-free translation system

Huong Thi Bui, Hiroshi Umakoshi, Keishi Suga, Tomoyuki Tanabe, Kien Xuan Ngo, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effect of cationic liposome prepared from 1,2-dioleoyl-3-trimethyl- ammonium-propane (DOTAP) on the gene expression at the mRNA translation level was investigated using an E. coli cell-free translation system. DOTAP liposome at 3 mM inhibited the mRNA translation of green fluorescent protein (GFP), as indicated both by the fluorescence intensity of GFP and by SDS-PAGE analysis. The role of DOTAP liposome on the inhibition of mRNA translation was revealed that the cationic quaternary amine groups on the liposome surface can interact and neutralize the anionic phosphate groups on mRNA by an electrostatic interaction. mRNA molecules still existed without any degradation in the presence of DOTAP liposome although it could not be translated. These results clearly illustrated that the DOTAP liposome could knock down mRNA and silence its activity of translation in an E. coli cell-free system.

Original languageEnglish
Pages (from-to)38-43
Number of pages6
JournalBiochemical Engineering Journal
Volume52
Issue number1
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Cell-Free System
Liposomes
Protein Biosynthesis
Propane
Escherichia coli
Ammonium Compounds
Messenger RNA
Green Fluorescent Proteins
Proteins
Coulomb interactions
Static Electricity
Gene expression
Amines
Polyacrylamide Gel Electrophoresis
Phosphates
Fluorescence
Gene Expression
Degradation
Molecules

Keywords

  • Cell free
  • GFP
  • Liposome
  • Membrane stress biotechnology
  • MRNA
  • Translation system

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

Cite this

Cationic liposome can interfere mRNA translation in an E. coli cell-free translation system. / Bui, Huong Thi; Umakoshi, Hiroshi; Suga, Keishi; Tanabe, Tomoyuki; Ngo, Kien Xuan; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Biochemical Engineering Journal, Vol. 52, No. 1, 10.2010, p. 38-43.

Research output: Contribution to journalArticle

Bui, Huong Thi ; Umakoshi, Hiroshi ; Suga, Keishi ; Tanabe, Tomoyuki ; Ngo, Kien Xuan ; Shimanouchi, Toshinori ; Kuboi, Ryoichi. / Cationic liposome can interfere mRNA translation in an E. coli cell-free translation system. In: Biochemical Engineering Journal. 2010 ; Vol. 52, No. 1. pp. 38-43.
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