Catheter-based prostacyclin synthase gene transfer prevents in-stent restenosis in rabbit atheromatous arteries

Yasushi Numaguchi, Kenji Okumura, Mitsunori Harada, Keiji Naruse, Michiharu Yamada, Hiroyuki Osanai, Hideo Matsui, Masafumi Ito, Toyoaki Murohara

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective: Prostacyclin synthase (PGIS) gene transfer have been shown to accelerate re-endothelialization and prevent neointimal formation in balloon-injured arteries. The aim of this study is to evaluate how overexpression of endogenous prostacyclin exerts those beneficial effects in atheromatous arteries. Methods: New Zealand White Rabbits fed a 0.5% cholesterol diet underwent balloon injury and Palmaz-Schatz stent implantation in the iliac arteries followed PGIS gene (pCMV-PGIS, 200 μg) delivery by the lipotransfection method via Dispatch catheter (n=6 each). Results: One week after transfection, arterial segments of pCMV-PGIS produced higher levels of 6-keto-PGF1α than those of control, pCMV-LacZ (p

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalCardiovascular Research
Volume61
Issue number1
DOIs
Publication statusPublished - Jan 1 2004
Externally publishedYes

Fingerprint

Stents
Catheters
Arteries
Rabbits
Iliac Artery
Epoprostenol
Genes
Transfection
Cholesterol
Diet
Wounds and Injuries
prostacyclin synthetase
prostaglandin F1

Keywords

  • Atherosclerosis
  • Gene therapy
  • Prostaglandins
  • Restenosis
  • Stents

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Catheter-based prostacyclin synthase gene transfer prevents in-stent restenosis in rabbit atheromatous arteries. / Numaguchi, Yasushi; Okumura, Kenji; Harada, Mitsunori; Naruse, Keiji; Yamada, Michiharu; Osanai, Hiroyuki; Matsui, Hideo; Ito, Masafumi; Murohara, Toyoaki.

In: Cardiovascular Research, Vol. 61, No. 1, 01.01.2004, p. 177-185.

Research output: Contribution to journalArticle

Numaguchi, Y, Okumura, K, Harada, M, Naruse, K, Yamada, M, Osanai, H, Matsui, H, Ito, M & Murohara, T 2004, 'Catheter-based prostacyclin synthase gene transfer prevents in-stent restenosis in rabbit atheromatous arteries', Cardiovascular Research, vol. 61, no. 1, pp. 177-185. https://doi.org/10.1016/j.cardiores.2003.10.016
Numaguchi, Yasushi ; Okumura, Kenji ; Harada, Mitsunori ; Naruse, Keiji ; Yamada, Michiharu ; Osanai, Hiroyuki ; Matsui, Hideo ; Ito, Masafumi ; Murohara, Toyoaki. / Catheter-based prostacyclin synthase gene transfer prevents in-stent restenosis in rabbit atheromatous arteries. In: Cardiovascular Research. 2004 ; Vol. 61, No. 1. pp. 177-185.
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AU - Matsui, Hideo

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