Cathepsin E deficiency induces a novel form of lysosomal storage disorder showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH in macrophages

Michiyo Yanagawa, Takayuki Tsukuba, Tsuyoshi Nishioku, Yoshiko Okamoto, Kuniaki Okamoto, Ryosuke Takii, Yoshihiro Terada, Keiichi I. Nakayama, Tomoko Kadowaki, Kenji Yamamoto

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Cathepsin E, an endolysosomal aspartic proteinase predominantly expressed in cells of the immune system, has an important role in immune responses. However, little is known about the precise roles of cathepsin E in this system. Here we report that cathepsin E deficiency (CatE-/-) leads to a novel form of lysosome storage disorder in macrophages, exhibiting the accumulation of the two major lysosomal membrane sialoglycoproteins LAMP-1 and LAMP-2 and the elevation of lysosomal pH. These striking features were also found in wild-type macrophages treated with pepstatin A and Ascaris inhibitor. Whereas there were no obvious differences in their expression, biosynthesis, and trafficking between wild-type and CatE-/- macrophages, the degradation rates of these two membrane proteins were apparently decreased as a result of cathepsin E deficiency. Because there was no difference in the vacuolar-type H +-ATPase activity in both cell types, the elevated lysosomal pH in CatE-/- macrophages is most likely due to the accumulation of these lysosomal membrane glycoproteins highly modified with acidic monosaccharides, thereby leading to the disruption of non-proton factors controlling lysosomal pH. Furthermore, the selective degradation of LAMP-1 and LAMP-2, as well as LIMP-2, was also observed by treatment of the lysosomal membrane fraction isolated from wild-type macrophages with purified cathepsin E at pH 5. Our results thus suggest that cathepsin E is important for preventing the accumulation of these lysosomal membrane sialoglycoproteins that can induce a new form of lysosomal storage disorder.

Original languageEnglish
Pages (from-to)1851-1862
Number of pages12
JournalJournal of Biological Chemistry
Volume282
Issue number3
DOIs
Publication statusPublished - Jan 19 2007
Externally publishedYes

Fingerprint

Cathepsin E
Sialoglycoproteins
Macrophages
Membranes
Lysosome-Associated Membrane Glycoproteins
Aspartic Acid Proteases
Vacuolar Proton-Translocating ATPases
Ascaris
Degradation
Monosaccharides
Immune system
Biosynthesis
Lysosomes
Immune System
Membrane Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cathepsin E deficiency induces a novel form of lysosomal storage disorder showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH in macrophages. / Yanagawa, Michiyo; Tsukuba, Takayuki; Nishioku, Tsuyoshi; Okamoto, Yoshiko; Okamoto, Kuniaki; Takii, Ryosuke; Terada, Yoshihiro; Nakayama, Keiichi I.; Kadowaki, Tomoko; Yamamoto, Kenji.

In: Journal of Biological Chemistry, Vol. 282, No. 3, 19.01.2007, p. 1851-1862.

Research output: Contribution to journalArticle

Yanagawa, Michiyo ; Tsukuba, Takayuki ; Nishioku, Tsuyoshi ; Okamoto, Yoshiko ; Okamoto, Kuniaki ; Takii, Ryosuke ; Terada, Yoshihiro ; Nakayama, Keiichi I. ; Kadowaki, Tomoko ; Yamamoto, Kenji. / Cathepsin E deficiency induces a novel form of lysosomal storage disorder showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH in macrophages. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 3. pp. 1851-1862.
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AU - Nishioku, Tsuyoshi

AU - Okamoto, Yoshiko

AU - Okamoto, Kuniaki

AU - Takii, Ryosuke

AU - Terada, Yoshihiro

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AU - Yamamoto, Kenji

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