Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands

Shin Morizane, Kenshi Yamasaki, Beda Mühleisen, Paul F. Kotol, Masamoto Murakami, Yumi Aoyama, Keiji Iwatsuki, Tissa Hata, Richard L. Gallo

Research output: Contribution to journalArticle

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Abstract

Here we show that keratinocytes in psoriatic lesional skin express increased Toll-like receptor (TLR) 9 that similarly localizes with elevated expression of the cathelicidin antimicrobial peptide LL-37. In culture, normal human keratinocytes exposed to LL-37 increased TLR9 expression. Furthermore, when keratinocytes were exposed to LL-37 and subsequently treated with TLR9 ligands, such as CpG or genomic DNA, they greatly increased production of type I IFNs. This response mimicked observations in the epidermis of psoriatic lesional skin as keratinocytes in psoriatic lesions produce greater amounts of IFN-Β than normal skin lacking LL-37. The mechanism for induction of type I IFNs in keratinocytes was dependent on TLR9 expression but not on a DNA-LL-37 complex. These findings suggest that keratinocytes recognize and respond to DNA and can actively participate in contributing to the immunological environment that characterizes psoriasis.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalJournal of Investigative Dermatology
Volume132
Issue number1
DOIs
Publication statusPublished - Jan 2012

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Keratinocytes
Psoriasis
Ligands
Skin
DNA
Toll-Like Receptor 9
Epidermis
cathelicidin antimicrobial peptide
antimicrobial peptide LL-37

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands. / Morizane, Shin; Yamasaki, Kenshi; Mühleisen, Beda; Kotol, Paul F.; Murakami, Masamoto; Aoyama, Yumi; Iwatsuki, Keiji; Hata, Tissa; Gallo, Richard L.

In: Journal of Investigative Dermatology, Vol. 132, No. 1, 01.2012, p. 135-143.

Research output: Contribution to journalArticle

Morizane, S, Yamasaki, K, Mühleisen, B, Kotol, PF, Murakami, M, Aoyama, Y, Iwatsuki, K, Hata, T & Gallo, RL 2012, 'Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands', Journal of Investigative Dermatology, vol. 132, no. 1, pp. 135-143. https://doi.org/10.1038/jid.2011.259
Morizane, Shin ; Yamasaki, Kenshi ; Mühleisen, Beda ; Kotol, Paul F. ; Murakami, Masamoto ; Aoyama, Yumi ; Iwatsuki, Keiji ; Hata, Tissa ; Gallo, Richard L. / Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands. In: Journal of Investigative Dermatology. 2012 ; Vol. 132, No. 1. pp. 135-143.
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