Catalytic properties of novel reversed micellar system on trans-esterification by α-chymotrypsin in organic media

Ly Iskandar, Tsutomu Ono, Noriho Kamiya, Masahiro Goto, Fumiyuki Nakashio, Shintaro Furusaki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Novel reversed micelles formulated by sodium dioleylphosphate (SDOLP) has been developed for enzymatic reaction in organic media. Comparison study between sodium bis-(2-ethylhexyl)-sulfosuccinate (Aerosol-OT or AOT) and SDOLP, which was carried out by investigating the reactivity of each reversed micellar system as reaction media for enzymatic transesterification reaction of N-acetyl-1-phenylalanine ethyl ester (APEE) and 1-propanol, leads to the upshot that α-chymotrypsin immobilized within SDOLP reversed micelles can persevere its high catalytic activity in isooctane. The initial reaction rate of the SDOLP reversed micellar system is almost 60 times higher than that in the AOT system. Additional kinetic results, including the Michaelis constant and the maximum reaction rate, reveal that the inner core of the SDOLP reversed micelles provides a better environment for enzymatic reaction than that of the conventional AOT reversed micelles.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalBiochemical Engineering Journal
Volume2
Issue number1
Publication statusPublished - Sep 1998
Externally publishedYes

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Keywords

  • AOT
  • Biocatalyst
  • Enzymatic reaction
  • Organic solvents
  • Reversed micelles

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Bioengineering

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