Catalytic capabilities of lipase immobilized into organically modified silicates by sol-gel method

Shin Ya Furukawa, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The capabilities of Candida rugosa lipase, which was immobilized into organically modified silicates formed on kieselguhr such as Celite 545 are investigated in esterification of (-)- or (+)-menthol with butyric acid. In this study, we optimized the preparation conditions such as a molar ratio of precursors. From these investigations, we find that the optimized hybrid gel-immobilized lipase is derived from a mixture of dimethyldimethoxysilane and tetramethoxysilane at a molar ratio of 4: 1 and its enantioselectivity, which is defined as a ratio of the initial reaction rate of (-)-menthol to that of (+) -menthol, is enhanced up to 41 at 75°C. In addition, we observe the temperature dependence of these immobilized lipases. Consequently, the optimized hybrid gel-immobilized lipase shows higher activity than deposited lipase on Celite at all temperatures, and it is suggested that this activation results from a remarkable enhancement of the V max value.

Original languageEnglish
Pages (from-to)885-886
Number of pages2
JournalKagaku Kogaku Ronbunshu
Volume26
Issue number6
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Silicates
Lipases
Lipase
Sol-gel process
Diatomaceous Earth
Menthol
Gels
Butyric acid
Butyric Acid
Candida
Enantioselectivity
Esterification
Reaction rates
Chemical activation
Temperature

Keywords

  • Enantioselective esterification
  • Lipase
  • Menthol
  • Organic-inorganic hybrid silicate
  • Sol-gel method

ASJC Scopus subject areas

  • Materials Science (miscellaneous)

Cite this

Catalytic capabilities of lipase immobilized into organically modified silicates by sol-gel method. / Furukawa, Shin Ya; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Koei.

In: Kagaku Kogaku Ronbunshu, Vol. 26, No. 6, 2000, p. 885-886.

Research output: Contribution to journalArticle

Furukawa, Shin Ya ; Ono, Tsutomu ; Ijima, Hiroyuki ; Kawakami, Koei. / Catalytic capabilities of lipase immobilized into organically modified silicates by sol-gel method. In: Kagaku Kogaku Ronbunshu. 2000 ; Vol. 26, No. 6. pp. 885-886.
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