Catalase catalyzes nitrotyrosine formation from sodium azide and hydrogen peroxide

Keiki Ogino, Norio Kodama, Madoka Nakajima, Akihiro Yamada, Hiroyuki Nakamura, Hirohumi Nagase, Daikai Sadamitsu, Takeshi Maekawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Sodium azide (NaN3) is known as an inhibitor of catalase, and a nitric oxide (NO) donor in the presence of catalase and H2O2. We showed here that catalase-catalyzed oxidation of NaN3 can generate reactive nitrogen species which contribute to tyrosine nitration in the presence of H2O2. The formation of free-tyrosine nitration and protein-bound tyrosine nitration by the NaN3/catalase/H2O2 system showed a maximum level at pH 6.0. Free-tyrosine nitration induced by peroxynitrite was inhibited by ethanol and dimethyl-sulfoxide (DMSO), and augmented by superoxide dismutase (SOD). However, free-tyrosine nitration induced by the NaN3/catalase/H2O2 system was not affected by ethanol, DMSO and SOD. NO2 and NO donating agents did not affect free-tyrosine nitration by the NaN3/catalase/H2O2 system. The reaction of NaN3 with hydroxyl radical generating system showed free-tyrosine nitration, but no formation of nitrite and nitrate. The generation of nitrite (NO2-) and nitrate (NO3-) by the NaN3/catalase/H2O2 system was maximal at pH 5.0. These results suggested that the oxidation of NaN3 by the catalase/H2O2 system generates unknown peroxynitrite-like reactive nitrogen intermediates, which contribute to tyrosine nitration.

Original languageEnglish
Pages (from-to)735-747
Number of pages13
JournalFree Radical Research
Volume35
Issue number6
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Sodium Azide
Nitration
Catalase
Hydrogen Peroxide
Tyrosine
Peroxynitrous Acid
Nitrites
Dimethyl Sulfoxide
Nitrates
Superoxide Dismutase
Ethanol
3-nitrotyrosine
sodium peroxide
Reactive Nitrogen Species
Oxidation
Nitric Oxide Donors
Hydroxyl Radical
Nitric Oxide
Nitrogen

Keywords

  • Azidyl radical
  • Catalase
  • Nitrotyrosine
  • Peroxynitrite
  • Sodium azide

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ogino, K., Kodama, N., Nakajima, M., Yamada, A., Nakamura, H., Nagase, H., ... Maekawa, T. (2001). Catalase catalyzes nitrotyrosine formation from sodium azide and hydrogen peroxide. Free Radical Research, 35(6), 735-747.

Catalase catalyzes nitrotyrosine formation from sodium azide and hydrogen peroxide. / Ogino, Keiki; Kodama, Norio; Nakajima, Madoka; Yamada, Akihiro; Nakamura, Hiroyuki; Nagase, Hirohumi; Sadamitsu, Daikai; Maekawa, Takeshi.

In: Free Radical Research, Vol. 35, No. 6, 2001, p. 735-747.

Research output: Contribution to journalArticle

Ogino, K, Kodama, N, Nakajima, M, Yamada, A, Nakamura, H, Nagase, H, Sadamitsu, D & Maekawa, T 2001, 'Catalase catalyzes nitrotyrosine formation from sodium azide and hydrogen peroxide', Free Radical Research, vol. 35, no. 6, pp. 735-747.
Ogino K, Kodama N, Nakajima M, Yamada A, Nakamura H, Nagase H et al. Catalase catalyzes nitrotyrosine formation from sodium azide and hydrogen peroxide. Free Radical Research. 2001;35(6):735-747.
Ogino, Keiki ; Kodama, Norio ; Nakajima, Madoka ; Yamada, Akihiro ; Nakamura, Hiroyuki ; Nagase, Hirohumi ; Sadamitsu, Daikai ; Maekawa, Takeshi. / Catalase catalyzes nitrotyrosine formation from sodium azide and hydrogen peroxide. In: Free Radical Research. 2001 ; Vol. 35, No. 6. pp. 735-747.
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AU - Nagase, Hirohumi

AU - Sadamitsu, Daikai

AU - Maekawa, Takeshi

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