Ca²⁺ signaling and homeostasis in mammalian oocytes and eggs

Changes in the intracellular concentration of calcium ([Ca²⁺]_i) represent a vital signaling mechanism enabling communication between and among cells as well as with the environment. Cells have developed a sophisticated set of molecules, “the Ca²⁺ toolkit,” to adapt [Ca²⁺]_i changes to specific cellular functions. Mammalian oocytes and eggs, the subject of this review, are not an exception, and in fact the initiation of embryo devolvement in all species is entirely dependent on distinct [Ca²⁺]_i responses. Here, we review the components of the Ca²⁺ toolkit present in mammalian oocytes and eggs, the regulatory mechanisms that allow these cells to accumulate Ca²⁺ in the endoplasmic reticulum, release it, and maintain basal and stable cytoplasmic concentrations. We also discuss electrophysiological and genetic studies that have uncovered Ca²⁺ influx channels in oocytes and eggs, and we analyze evidence supporting the role of a sperm-specific phospholipase C isoform as the trigger of Ca²⁺ oscillations during mammalian fertilization including its implication in fertility.