TY - JOUR
T1 - Ca2+ regulation of outward rectifying K+ channel in the plasma membrane of tobacco cultured cells in suspension
T2 - A role of the K+ channel in mitigation of salt-stress effects by external Ca2+
AU - Murata, Yoshiyuki
AU - Yoshihashi, Manabu
AU - Obi, Ichiro
AU - Kakutani, Tadaaki
PY - 1998/10
Y1 - 1998/10
N2 - The patch-clamp technique was used to study effect of the Ca2+ on K+ channels in the plasma membrane of protoplasts isolated from tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells in suspension. The outward rectifying whole-cell K+ currents were not affected by intracellular Ca2+, but they were reduced with increasing extracellular Ca2+. Neither extracellular nor intracellular Ca2+ affected the permeability ratios (P(K)+/P(Na)+) of the plasma membrane. These results suggest that the inhibition of outward-rectifying K+ channels by extracellular Ca2+ may partially contribute towards the mitigation of detrimental effects of salinity on growth by extracellular Ca2+.
AB - The patch-clamp technique was used to study effect of the Ca2+ on K+ channels in the plasma membrane of protoplasts isolated from tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells in suspension. The outward rectifying whole-cell K+ currents were not affected by intracellular Ca2+, but they were reduced with increasing extracellular Ca2+. Neither extracellular nor intracellular Ca2+ affected the permeability ratios (P(K)+/P(Na)+) of the plasma membrane. These results suggest that the inhibition of outward-rectifying K+ channels by extracellular Ca2+ may partially contribute towards the mitigation of detrimental effects of salinity on growth by extracellular Ca2+.
KW - Ca mitigation of salt stress
KW - Nicotiana tabacum L.
KW - Outward rectifying K channel
KW - Patch clamp
KW - Plasma membrane
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U2 - 10.1093/oxfordjournals.pcp.a029300
DO - 10.1093/oxfordjournals.pcp.a029300
M3 - Article
AN - SCOPUS:0031789875
VL - 39
SP - 1039
EP - 1044
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 10
ER -