Ca2+ regulation of outward rectifying K+ channel in the plasma membrane of tobacco cultured cells in suspension: A role of the K+ channel in mitigation of salt-stress effects by external Ca2+

Yoshiyuki Murata, Manabu Yoshihashi, Ichiro Obi, Tadaaki Kakutani

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The patch-clamp technique was used to study effect of the Ca2+ on K+ channels in the plasma membrane of protoplasts isolated from tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells in suspension. The outward rectifying whole-cell K+ currents were not affected by intracellular Ca2+, but they were reduced with increasing extracellular Ca2+. Neither extracellular nor intracellular Ca2+ affected the permeability ratios (P(K)+/P(Na)+) of the plasma membrane. These results suggest that the inhibition of outward-rectifying K+ channels by extracellular Ca2+ may partially contribute towards the mitigation of detrimental effects of salinity on growth by extracellular Ca2+.

Original languageEnglish
Pages (from-to)1039-1044
Number of pages6
JournalPlant and Cell Physiology
Issue number10
Publication statusPublished - Oct 1998



  • Ca mitigation of salt stress
  • Nicotiana tabacum L.
  • Outward rectifying K channel
  • Patch clamp
  • Plasma membrane

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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