Castration-induced changes in mouse epididymal white adipose tissue

Daniel Floryk, Shinji Kurosaka, Ryuta Tanimoto, Guang Yang, Alexei Goltsov, Sanghee Park, Timothy C. Thompson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We analyzed the effects of castration on epididymal white adipose tissue (WAT) in C57BL/6J mice which were fed a regular or high-fat diet. Fourteen days following surgical castration profound effects on WAT tissue such as reductions in WAT wet weight and WAT/body weight ratio, induction of lipolysis and morphologic changes characterized by smaller adipocytes, and increased stromal cell compartment were documented in both dietary groups. Castrated animals had decreased serum leptin levels independent of diet but diet-dependent decreases in serum adiponectin and resistin. The castrated high-fat group had dramatically lower serum triglyceride levels. Immunohistochemical analysis revealed higher staining for smooth muscle actin, macrophage marker Mac-3, and Cxcl5 in the castrated than in the control mice in both dietary groups. We also detected increased fatty-acid synthase expression in the stromal compartment of WAT in the regular-diet group. Castration also reduces the expression of androgen receptor in WAT in the regular-diet group. We conclude that castration reduces tissue mass and affects biologic function of WAT in mice.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume345
Issue number1-2
DOIs
Publication statusPublished - Oct 15 2011
Externally publishedYes

Fingerprint

White Adipose Tissue
Castration
Tissue
Nutrition
Diet
Serum
Resistin
Fatty Acid Synthases
Fats
Lipolysis
Adiponectin
Androgen Receptors
High Fat Diet
Stromal Cells
Leptin
Inbred C57BL Mouse
Adipocytes
Macrophages
Smooth Muscle
Actins

Keywords

  • C57BL/6J mouse
  • Castration
  • Epididymal white adipose tissue
  • High-fat diet
  • Regular diet

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Floryk, D., Kurosaka, S., Tanimoto, R., Yang, G., Goltsov, A., Park, S., & Thompson, T. C. (2011). Castration-induced changes in mouse epididymal white adipose tissue. Molecular and Cellular Endocrinology, 345(1-2), 58-67. https://doi.org/10.1016/j.mce.2011.07.011

Castration-induced changes in mouse epididymal white adipose tissue. / Floryk, Daniel; Kurosaka, Shinji; Tanimoto, Ryuta; Yang, Guang; Goltsov, Alexei; Park, Sanghee; Thompson, Timothy C.

In: Molecular and Cellular Endocrinology, Vol. 345, No. 1-2, 15.10.2011, p. 58-67.

Research output: Contribution to journalArticle

Floryk, D, Kurosaka, S, Tanimoto, R, Yang, G, Goltsov, A, Park, S & Thompson, TC 2011, 'Castration-induced changes in mouse epididymal white adipose tissue', Molecular and Cellular Endocrinology, vol. 345, no. 1-2, pp. 58-67. https://doi.org/10.1016/j.mce.2011.07.011
Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S et al. Castration-induced changes in mouse epididymal white adipose tissue. Molecular and Cellular Endocrinology. 2011 Oct 15;345(1-2):58-67. https://doi.org/10.1016/j.mce.2011.07.011
Floryk, Daniel ; Kurosaka, Shinji ; Tanimoto, Ryuta ; Yang, Guang ; Goltsov, Alexei ; Park, Sanghee ; Thompson, Timothy C. / Castration-induced changes in mouse epididymal white adipose tissue. In: Molecular and Cellular Endocrinology. 2011 ; Vol. 345, No. 1-2. pp. 58-67.
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