Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray

Yoshinori Utsumi, Maho Tanaka, Atsushi Kurotani, Takuhiro Yoshida, Keiichi Mochida, Akihiro Matsui, Manabu Ishitani, Supajit Sraphet, Sukhuman Whankaew, Thipa Asvarak, Jarunya Narangajavana, Kanokporn Triwitayakorn, Tetsuya Sakurai, Motoaki Seki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cassava anthracnose disease (CAD), caused by the fungus Colletotrichumgloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tags and RNA-seq information from cassava, and applied it to investigate the molecular mechanisms of resistance to fungal infection using two cassava cultivars, Huay Bong 60 (HB60, resistant to CAD) and Hanatee (HN, sensitive to CAD). Based on quantitative real-time reverse transcription PCR and expression profiling by the microarray, we showed that the expressions of various plant defense-related genes, such as pathogenesis-related (PR) genes, cell wall-related genes, detoxification enzyme, genes related to the response to bacterium, mitogen-activated protein kinase (MAPK), genes related to salicylic acid, jasmonic acid and ethylene pathways were higher in HB60 compared with HN. Our results indicated that the induction of PR genes in HB60 by fungal infection and the higher expressions of defense response-related genes in HB60 compared with HN are likely responsible for the fungal resistance in HB60. We also showed that the use of our cassava oligo microarray could improve our understanding of cassava molecular mechanisms related to environmental responses and development, and advance the molecular breeding of useful cassava plants.

Original languageEnglish
Pages (from-to)711-726
Number of pages16
JournalJournal of Plant Research
Volume129
Issue number4
DOIs
Publication statusPublished - Jul 1 2016
Externally publishedYes

Fingerprint

Glomerella cingulata
Manihot esculenta
oligonucleotides
transcriptomics
cassava
fungi
infection
anthracnose
genes
pathogenesis
DNA microarrays
jasmonic acid
expressed sequence tags
resistance mechanisms
mitogen-activated protein kinase
salicylic acid
ethylene
reverse transcriptase polymerase chain reaction
cell walls
RNA

Keywords

  • Cassava
  • Cassava anthracnose disease
  • Detoxification
  • Disease resistance
  • Transcriptome

ASJC Scopus subject areas

  • Plant Science

Cite this

Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray. / Utsumi, Yoshinori; Tanaka, Maho; Kurotani, Atsushi; Yoshida, Takuhiro; Mochida, Keiichi; Matsui, Akihiro; Ishitani, Manabu; Sraphet, Supajit; Whankaew, Sukhuman; Asvarak, Thipa; Narangajavana, Jarunya; Triwitayakorn, Kanokporn; Sakurai, Tetsuya; Seki, Motoaki.

In: Journal of Plant Research, Vol. 129, No. 4, 01.07.2016, p. 711-726.

Research output: Contribution to journalArticle

Utsumi, Y, Tanaka, M, Kurotani, A, Yoshida, T, Mochida, K, Matsui, A, Ishitani, M, Sraphet, S, Whankaew, S, Asvarak, T, Narangajavana, J, Triwitayakorn, K, Sakurai, T & Seki, M 2016, 'Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray', Journal of Plant Research, vol. 129, no. 4, pp. 711-726. https://doi.org/10.1007/s10265-016-0828-x
Utsumi, Yoshinori ; Tanaka, Maho ; Kurotani, Atsushi ; Yoshida, Takuhiro ; Mochida, Keiichi ; Matsui, Akihiro ; Ishitani, Manabu ; Sraphet, Supajit ; Whankaew, Sukhuman ; Asvarak, Thipa ; Narangajavana, Jarunya ; Triwitayakorn, Kanokporn ; Sakurai, Tetsuya ; Seki, Motoaki. / Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray. In: Journal of Plant Research. 2016 ; Vol. 129, No. 4. pp. 711-726.
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AU - Yoshida, Takuhiro

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AU - Asvarak, Thipa

AU - Narangajavana, Jarunya

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