Casein kinase 2 phosphorylates and stabilizes C/EBPβ in pancreatic β cells

Tomoko Takai, Tomokazu Matsuda, Yuki Matsuura, Kaho Inoue, Emi Suzuki, Ayumi Kanno, Maki Kimura-Koyanagi, Shun ichiro Asahara, Naoya Hatano, Wataru Ogawa, Yoshiaki Kido

Research output: Contribution to journalArticle

Abstract

During the development of type 2 diabetes, endoplasmic reticulum (ER) stress leads to pancreatic β cell failure. CCAAT/enhancer-binding protein (C/EBP) β is highly induced by ER stress and AMP-activated protein kinase (AMPK) suppression in pancreatic β cells, and its accumulation reduces pancreatic β cell mass. We investigated the phosphorylation state of C/EBPβ under these conditions. Casein kinase 2 (CK2) was found to co-localize with C/EBPβ in MIN6 cells. It phosphorylated S222 of C/EBPβ a previously unidentified phosphorylation site. We found that C/EBPβ is phosphorylated by CK2 under AMPK suppression and ER stress, which are important from the viewpoint of the worsening pathological condition of type 2 diabetes, such as decreased insulin secretion and apoptosis of pancreatic β cells.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume497
Issue number1
DOIs
Publication statusPublished - Feb 26 2018
Externally publishedYes

Fingerprint

CCAAT-Enhancer-Binding Proteins
Casein Kinase II
Endoplasmic Reticulum Stress
Phosphorylation
AMP-Activated Protein Kinases
Medical problems
ditazol
Type 2 Diabetes Mellitus
Insulin
Apoptosis

Keywords

  • Casein kinase 2 (CK2)
  • CCAAT/Enhancer-binding protein β (C/EBPβ)
  • Endoplasmic reticulum stress
  • Pancreatic β cell failure

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Takai, T., Matsuda, T., Matsuura, Y., Inoue, K., Suzuki, E., Kanno, A., ... Kido, Y. (2018). Casein kinase 2 phosphorylates and stabilizes C/EBPβ in pancreatic β cells. Biochemical and Biophysical Research Communications, 497(1), 451-456. https://doi.org/10.1016/j.bbrc.2018.02.108

Casein kinase 2 phosphorylates and stabilizes C/EBPβ in pancreatic β cells. / Takai, Tomoko; Matsuda, Tomokazu; Matsuura, Yuki; Inoue, Kaho; Suzuki, Emi; Kanno, Ayumi; Kimura-Koyanagi, Maki; Asahara, Shun ichiro; Hatano, Naoya; Ogawa, Wataru; Kido, Yoshiaki.

In: Biochemical and Biophysical Research Communications, Vol. 497, No. 1, 26.02.2018, p. 451-456.

Research output: Contribution to journalArticle

Takai, T, Matsuda, T, Matsuura, Y, Inoue, K, Suzuki, E, Kanno, A, Kimura-Koyanagi, M, Asahara, SI, Hatano, N, Ogawa, W & Kido, Y 2018, 'Casein kinase 2 phosphorylates and stabilizes C/EBPβ in pancreatic β cells', Biochemical and Biophysical Research Communications, vol. 497, no. 1, pp. 451-456. https://doi.org/10.1016/j.bbrc.2018.02.108
Takai, Tomoko ; Matsuda, Tomokazu ; Matsuura, Yuki ; Inoue, Kaho ; Suzuki, Emi ; Kanno, Ayumi ; Kimura-Koyanagi, Maki ; Asahara, Shun ichiro ; Hatano, Naoya ; Ogawa, Wataru ; Kido, Yoshiaki. / Casein kinase 2 phosphorylates and stabilizes C/EBPβ in pancreatic β cells. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 497, No. 1. pp. 451-456.
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