Casein Enhances Stability of Peptides in Intestinal Lumen: Role of Digested Products of Casein

Seiji Ohtani, Ken Ichi Ogawara, Kazutaka Higaki, Toshikiro Kimura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose. To investigate the inhibitory activity of casein on proteases in detail, the effect of digested products of casein itself on trypsin and chymotrypsin in rat small intestine was examined. Methods. Male Sprague-Dawley rats weighing 200-300 g were used as the animal model. The luminal content of the jejunum was prepared, and the enzymatic activities of trypsin and chymotrypsin were determined using a specific substrate for each protease. Then, the effect of enzymatic digested products of casein on them was examined. Results. The inhibitory activity of trypsin-digested casein against trypsin decreased as its digestion proceeded, but its inhibitory activity against chymotrypsin came to be more effective. On the other hand, the inhibitory activity of chymotrypsin-digested casein against chymotrypsin decreased with the degree of digestion, but no change in the inhibitory activity against trypsin was observed. Even the completely digested products of casein with trypsin or chymotrypsin showed inhibitory activities against the two proteases. Conclusions. It was suggested that not only the intact casein but also the products digested with trypsin or chymotrypsin contribute to the inhibitory effect of casein on the proteases in the intestinal lumen.

Original languageEnglish
Pages (from-to)1746-1751
Number of pages6
JournalPharmaceutical Research
Volume20
Issue number11
DOIs
Publication statusPublished - Nov 2003

Fingerprint

Caseins
Chymotrypsin
Peptides
Trypsin
Peptide Hydrolases
Rats
Digestion
Weighing
Jejunum
Small Intestine
Sprague Dawley Rats
Animals
Animal Models
trypsin drug combination chymotrypsin
Substrates

Keywords

  • Casein
  • Digestion product
  • Enzyme inhibition
  • Protease
  • Small intestine

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmaceutical Science
  • Pharmacology

Cite this

Casein Enhances Stability of Peptides in Intestinal Lumen : Role of Digested Products of Casein. / Ohtani, Seiji; Ogawara, Ken Ichi; Higaki, Kazutaka; Kimura, Toshikiro.

In: Pharmaceutical Research, Vol. 20, No. 11, 11.2003, p. 1746-1751.

Research output: Contribution to journalArticle

Ohtani, Seiji ; Ogawara, Ken Ichi ; Higaki, Kazutaka ; Kimura, Toshikiro. / Casein Enhances Stability of Peptides in Intestinal Lumen : Role of Digested Products of Casein. In: Pharmaceutical Research. 2003 ; Vol. 20, No. 11. pp. 1746-1751.
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