Cartducin, a paralog of Acrp30/adiponectin, is induced during chondrogenic differentiation and promotes proliferation of chondrogenic precursors and chondrocytes

Takashi Maeda, Akitoshi Jikko, Makoto Abe, Tamaki Yokohama-Tamaki, Hironori Akiyama, Souhei Furukawa, Masaharu Takigawa, Satoshi Wakisaka

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We previously reported that CORS26 gene, isolated from C3H10T1/2 cells treated with transforming growth factor-β1, was predominantly expressed in cartilage. Because the gene product is a kind of secretory protein produced by cartilage tissue, we named it "cartducin". Cartducin shares a similar modular organization to adipocyte-derived hormone, adiponectin. In this study, we investigated cartducin function during chondrogenesis and cartilage development. In situ hybridization analysis showed that cartducin transcripts were restricted to the proliferating chondrocytes in the growth plate cartilage. Whole-mount in situ hybridization revealed that the first significant induction of cartducin expression occurred in the sclerotome, which contains a chondrogenic cell lineage between days 9.5 and 10.5 postcoitus (p.c.) during mouse embryogenesis. Chondrogenic differentiation by combined treatment with bone morphogenetic protein-2 and insulin induced cartducin expression along with type II and IX collagen expression in chondrogenic progenitor N1511 cells. To elucidate the direct action of cartducin on the cells, recombinant cartducin protein was expressed in and purified from Escherichia coli. The recombinant cartducin potentially forms homooligomers and promoted the proliferation of chondrogenic progenitor N1511 cells, and chondrocytic HCS-2/8 cells in a dose-dependent manner. On the other hand, cartducin did not affect the production of sulfated glycosaminoglycan (sGAG) in these cells. These findings indicate that cartducin is a novel growth factor and plays important roles in regulating both chondrogenesis and cartilage development by its direct stimulatory action on the proliferation of chondrogenic precursors and chondrocytes.

Original languageEnglish
Pages (from-to)537-544
Number of pages8
JournalJournal of Cellular Physiology
Volume206
Issue number2
DOIs
Publication statusPublished - Feb 2006

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Adiponectin
Cartilage
Chondrocytes
Chondrogenesis
A73025
In Situ Hybridization
Collagen Type IX
Stem Cells
Genes
Recombinant proteins
Bone Morphogenetic Protein 2
Growth Plate
Collagen Type II
Transforming Growth Factors
Cell Lineage
Recombinant Proteins
Adipocytes
Escherichia coli
Embryonic Development
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Cartducin, a paralog of Acrp30/adiponectin, is induced during chondrogenic differentiation and promotes proliferation of chondrogenic precursors and chondrocytes. / Maeda, Takashi; Jikko, Akitoshi; Abe, Makoto; Yokohama-Tamaki, Tamaki; Akiyama, Hironori; Furukawa, Souhei; Takigawa, Masaharu; Wakisaka, Satoshi.

In: Journal of Cellular Physiology, Vol. 206, No. 2, 02.2006, p. 537-544.

Research output: Contribution to journalArticle

Maeda, Takashi ; Jikko, Akitoshi ; Abe, Makoto ; Yokohama-Tamaki, Tamaki ; Akiyama, Hironori ; Furukawa, Souhei ; Takigawa, Masaharu ; Wakisaka, Satoshi. / Cartducin, a paralog of Acrp30/adiponectin, is induced during chondrogenic differentiation and promotes proliferation of chondrogenic precursors and chondrocytes. In: Journal of Cellular Physiology. 2006 ; Vol. 206, No. 2. pp. 537-544.
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