Carrier-mediated transport systems for glucose in mucosal cells of the human oral cavity

Yujiro Oyama, Hitoshi Yamano, Akiko Ohkuma, Ken Ichi Ogawara, Kazutaka Higaki, Toshikiro Kimura

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The in vitro uptake study was performed using the isolated cells of human oral mucosa, buccal and the dorsum of the tongue, to investigate the mechanisms of glucose uptake. The uptake of D-glucose was much larger in cells of the dorsum of the tongue than in buccal cells and was inhibited more extensively by 2-deoxy-o-glucose, a substrate of facilitative glucose transporters, than by α-methyl-D-glucoside, a specific substrate of SGLT1, suggesting the larger contribution of a facilitative transporter than Na+/glucose cotransporter. Furthermore, from the results of inhibition studies by several sugar analogue, including maltose and o-mannose, GLUT1 and/or GLUT3 were suggested to take part in the glucose uptake by oral mucosa. Therefore we have attempted to confirm the expression of glucose transporters on the oral mucosa by employing Western blotting. As a result, it was suggested that SGLT1, GLUT1, GLUT2, and GLUT3 are expressed in the epithelial cells of human oral mucosa.

Original languageEnglish
Pages (from-to)830-834
Number of pages5
JournalJournal of Pharmaceutical Sciences
Volume88
Issue number8
DOIs
Publication statusPublished - Aug 1999

ASJC Scopus subject areas

  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Carrier-mediated transport systems for glucose in mucosal cells of the human oral cavity'. Together they form a unique fingerprint.

  • Cite this