Caries inhibitory activity of cacao bean husk extract in in-vitro and animal experiments

T. Ooshima, Y. Osaka, H. Sasaki, K. Osawa, H. Yasuda, M. Matsumura, S. Sobue, M. Matsumoto

Research output: Contribution to journalArticlepeer-review

117 Citations (Scopus)


Cacao bean husk extract (CBH) was examined for inhibitory effects on the caries-inducing properties of mutans streptococci in vitro and on caries development in specific pathogen-free Sprague-Dawley rats infected with mutans streptococci. CBH reduced the growth rate of almost all oral streptococci examined, which resulted in the reduction of acid production. Furthermore, insoluble glucan synthesis by the glucosyltransferases from Streptococcus mutans MT8148R and Streptococcus sobrinus 6715 was significantly inhibited by CBH. Hence, the sucrose-dependent cell adherence of mutans streptococci was also depressed by CBH. The administration of CBH in drinking water resulted in significant reductions of caries development and dental plaque accumulation in rats infected with either Strep. sobrinus 6715 or Strep. mutans MT8148R, and the minimum cariostatic concentration was 1.0 mg/ml. These results indicate that CBH possesses powerful anticariogenic potential. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)639-645
Number of pages7
JournalArchives of Oral Biology
Issue number8
Publication statusPublished - Aug 1 2000
Externally publishedYes


  • Cacao bean husk
  • Dental caries
  • Enzyme inhibitors
  • Glucosyltransferase
  • Mutans streptococci

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology


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