Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction

Michio Nakaya, Kenji Watari, Mitsuru Tajima, Takeo Nakaya, Shoichi Matsuda, Hiroki Ohara, Hiroaki Nishihara, Hiroshi Yamaguchi, Akiko Hashimoto, Mitsuho Nishida, Akiomi Nagasaka, Yuma Horii, Hiroki Ono, Gentaro Iribe, Ryuji Inoue, Makoto Tsuda, Kazuhide Inoue, Akira Tanaka, Masahiko Kuroda, Shigekazu Nagata & 1 others Hitoshi Kurose

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Myocardial infarction (MI) results in the generation of dead cells in the infarcted area. These cells are swiftly removed by phagocytes to minimize inflammation and limit expansion of the damaged area. However, the types of cells and molecules responsible for the engulfment of dead cells in the infarcted area remain largely unknown. In this study, we demonstrated that cardiac myofibroblasts, which execute tissue fibrosis by producing extracellular matrix proteins, efficiently engulf dead cells. Furthermore, we identified a population of cardiac myofibroblasts that appears in the heart after MI in humans and mice. We found that these cardiac myofibroblasts secrete milk fat globule-epidermal growth factor 8 (MFG-E8), which promotes apoptotic engulfment, and determined that serum response factor is important for MFG-E8 production in myofibroblasts. Following MFG-E8-mediated engulfment of apoptotic cells, myofibroblasts acquired antiinflammatory properties. MFG-E8 deficiency in mice led to the accumulation of unengulfed dead cells after MI, resulting in exacerbated inflammatory responses and a substantial decrease in survival. Moreover, MFG-E8 administration into infarcted hearts restored cardiac function and morphology. MFG-E8-producing myofibroblasts mainly originated from resident cardiac fibroblasts and cells that underwent endothelial-mesenchymal transition in the heart. Together, our results reveal previously unrecognized roles of myofibroblasts in regulating apoptotic engulfment and a fundamental importance of these cells in recovery from MI.

Original languageEnglish
Pages (from-to)383-401
Number of pages19
JournalJournal of Clinical Investigation
Volume127
Issue number1
DOIs
Publication statusPublished - Jan 3 2017
Externally publishedYes

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Myofibroblasts
Myocardial Infarction
Epidermal Growth Factor
Serum Response Factor
Extracellular Matrix Proteins
Phagocytes
milk fat globule
Fibrosis
Anti-Inflammatory Agents
Endothelial Cells
Fibroblasts
Inflammation
Survival

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Nakaya, M., Watari, K., Tajima, M., Nakaya, T., Matsuda, S., Ohara, H., ... Kurose, H. (2017). Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. Journal of Clinical Investigation, 127(1), 383-401. https://doi.org/10.1172/JCI83822

Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. / Nakaya, Michio; Watari, Kenji; Tajima, Mitsuru; Nakaya, Takeo; Matsuda, Shoichi; Ohara, Hiroki; Nishihara, Hiroaki; Yamaguchi, Hiroshi; Hashimoto, Akiko; Nishida, Mitsuho; Nagasaka, Akiomi; Horii, Yuma; Ono, Hiroki; Iribe, Gentaro; Inoue, Ryuji; Tsuda, Makoto; Inoue, Kazuhide; Tanaka, Akira; Kuroda, Masahiko; Nagata, Shigekazu; Kurose, Hitoshi.

In: Journal of Clinical Investigation, Vol. 127, No. 1, 03.01.2017, p. 383-401.

Research output: Contribution to journalArticle

Nakaya, M, Watari, K, Tajima, M, Nakaya, T, Matsuda, S, Ohara, H, Nishihara, H, Yamaguchi, H, Hashimoto, A, Nishida, M, Nagasaka, A, Horii, Y, Ono, H, Iribe, G, Inoue, R, Tsuda, M, Inoue, K, Tanaka, A, Kuroda, M, Nagata, S & Kurose, H 2017, 'Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction', Journal of Clinical Investigation, vol. 127, no. 1, pp. 383-401. https://doi.org/10.1172/JCI83822
Nakaya, Michio ; Watari, Kenji ; Tajima, Mitsuru ; Nakaya, Takeo ; Matsuda, Shoichi ; Ohara, Hiroki ; Nishihara, Hiroaki ; Yamaguchi, Hiroshi ; Hashimoto, Akiko ; Nishida, Mitsuho ; Nagasaka, Akiomi ; Horii, Yuma ; Ono, Hiroki ; Iribe, Gentaro ; Inoue, Ryuji ; Tsuda, Makoto ; Inoue, Kazuhide ; Tanaka, Akira ; Kuroda, Masahiko ; Nagata, Shigekazu ; Kurose, Hitoshi. / Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. In: Journal of Clinical Investigation. 2017 ; Vol. 127, No. 1. pp. 383-401.
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