Carboxyl-terminal structure of basic fibroblast growth factor significantly contributes to its affinity for heparin

Masaharu Seno, R. Sasada, T. Kurokawa, K. Igarashi

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The carboxyl-terminal sequence of basic fibroblast growth factor (bFGF) is rich in basic amino acid residues, a common characteristic amongst fibroblast growth factors, and is considered to contribute greatly to the binding to negatively charged extracellular matrixes such as heparin. To study the relationship between the affinity for heparin and the carboxyl-terminal structure of bFGF, amino- or carboxyl-terminal truncated molecules were produced in Escherichia coli using recombinant DNA techniques. These terminally truncated bFGFs were applied to a heparin-affinity HPLC column. Truncation of more than six amino acid residues from the carboxyl-terminal made by the bFGF produced in E. coli marked difficult to solubilize and weakened its affinity for heparin, though bFGF having up to 46 amino acids removed showed significant stimulation of the DNA synthesis of BALB/c3T3 cells. This stimulation of the DNA synthesis was also recognized by the bFGF having 40 amino acids removed from its amino-terminal, while the affinity of this peptide for heparin has been shown to be equal to that of the mature bFGF (146 amino acids). These results show that the affinity of bFGF for heparin depends significantly on its carboxyl-terminal structure and that the essential part for receptor binding is present between Asp41 and Ser100. Moreover, it suggests that the Phe139 Leu140 Pro141, present in all members of the FGF family, contributes greatly to the stable structure of the intact molecule.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalEuropean Journal of Biochemistry
Volume188
Issue number2
Publication statusPublished - 1990
Externally publishedYes

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Fibroblast Growth Factor 2
Heparin
Amino Acids
Escherichia coli
Basic Amino Acids
Molecules
Recombinant DNA
Fibroblast Growth Factors
DNA
Extracellular Matrix
High Pressure Liquid Chromatography
Peptides

ASJC Scopus subject areas

  • Biochemistry

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Carboxyl-terminal structure of basic fibroblast growth factor significantly contributes to its affinity for heparin. / Seno, Masaharu; Sasada, R.; Kurokawa, T.; Igarashi, K.

In: European Journal of Biochemistry, Vol. 188, No. 2, 1990, p. 239-245.

Research output: Contribution to journalArticle

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