Carbon monoxide protects rat lung transplants from ischemia-reperfusion injury via a mechanism involving p38 MAPK pathway

J. Kohmoto, Atsunori Nakao, D. B. Stolz, T. Kaizu, A. Tsung, A. Ikeda, H. Shimizu, T. Takahashi, K. Tomiyama, R. Sugimoto, A. M K Choi, T. R. Billiar, N. Murase, K. R. McCurry

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Carbon monoxide (CO) provides protection against oxidative stress via anti-inflammatory and cytoprotective actions. In this study, we tested the hypothesis that a low concentration of exogenous (inhaled) CO would protect transplanted lung grafts from cold ischemia-reperfusion injury via a mechanism involving the mitogen-activated protein kinase (MAPK) signaling pathway. Lewis rats underwent orthotopic syngeneic or allogeneic left lung transplantation with 6 h of cold static preservation. Exposure of donors and recipients (1 h before and then continuously post-transplant) to 250 ppm CO resulted in significant improvement in gas exchange, reduced leukocyte sequestration, preservation of parenchymal and endothelial cell ultrastructure and reduced inflammation compared to animals exposed to air. The beneficial effects of CO were associated with p38 MAPK phosphorylation and were significantly prevented by treatment with a p38 MAPK inhibitor, suggesting that CO's efficacy is at least partially mediated by activation of p38 MAPK. Furthermore, CO markedly suppressed inflammatory events in the contralateral naïve lung. This study demonstrates that perioperative exposure of donors and recipients to CO at a low concentration can impart potent anti-inflammatory and cytoprotective effects in a clinically relevant model of lung transplantation and support further evaluation for potential clinical use.

Original languageEnglish
Pages (from-to)2279-2290
Number of pages12
JournalAmerican Journal of Transplantation
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Fingerprint

p38 Mitogen-Activated Protein Kinases
Carbon Monoxide
Reperfusion Injury
Transplants
Lung
Lung Transplantation
Anti-Inflammatory Agents
Cold Ischemia
Protein Kinase Inhibitors
Mitogen-Activated Protein Kinases
Oxidative Stress
Leukocytes
Endothelial Cells
Gases
Air
Phosphorylation
Inflammation

Keywords

  • Carbon monoxide
  • Heme oxygenase-1
  • Ischemia-reperfusion
  • Lung transplantation
  • p38 MAPK

ASJC Scopus subject areas

  • Immunology

Cite this

Carbon monoxide protects rat lung transplants from ischemia-reperfusion injury via a mechanism involving p38 MAPK pathway. / Kohmoto, J.; Nakao, Atsunori; Stolz, D. B.; Kaizu, T.; Tsung, A.; Ikeda, A.; Shimizu, H.; Takahashi, T.; Tomiyama, K.; Sugimoto, R.; Choi, A. M K; Billiar, T. R.; Murase, N.; McCurry, K. R.

In: American Journal of Transplantation, Vol. 7, No. 10, 10.2007, p. 2279-2290.

Research output: Contribution to journalArticle

Kohmoto, J, Nakao, A, Stolz, DB, Kaizu, T, Tsung, A, Ikeda, A, Shimizu, H, Takahashi, T, Tomiyama, K, Sugimoto, R, Choi, AMK, Billiar, TR, Murase, N & McCurry, KR 2007, 'Carbon monoxide protects rat lung transplants from ischemia-reperfusion injury via a mechanism involving p38 MAPK pathway', American Journal of Transplantation, vol. 7, no. 10, pp. 2279-2290. https://doi.org/10.1111/j.1600-6143.2007.01940.x
Kohmoto, J. ; Nakao, Atsunori ; Stolz, D. B. ; Kaizu, T. ; Tsung, A. ; Ikeda, A. ; Shimizu, H. ; Takahashi, T. ; Tomiyama, K. ; Sugimoto, R. ; Choi, A. M K ; Billiar, T. R. ; Murase, N. ; McCurry, K. R. / Carbon monoxide protects rat lung transplants from ischemia-reperfusion injury via a mechanism involving p38 MAPK pathway. In: American Journal of Transplantation. 2007 ; Vol. 7, No. 10. pp. 2279-2290.
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