Carbon monoxide differentially inhibits TLR signaling pathways by regulating ROS-induced trafficking of TLRs to lipid rafts

Kiichi Nakahira, Pyo Kim Hong, Hui Geng Xue, Atsunori Nakao, Xue Wang, Noriko Murase, Peter F. Drain, Xiaomei Wang, Madhu Sasidhar, Elizabeth G. Nabel, Toru Takahashi, Nicholas W. Lukacs, Stefan W. Ryter, Kiyoshi Morita, Augustine M K Choi

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Carbon monoxide (CO), a byproduct of heme catabolism by heme oxygenase (HO), confers potent antiinflammatory effects. Here we demonstrate that CO derived from HO-1 inhibited Toll-like receptor (TLR) 2, 4, 5, and 9 signaling, but not TLR3-dependent signaling, in macrophages. Ligand-mediated receptor trafficking to lipid rafts represents an early event in signal initiation of immune cells. Trafficking of TLR4 to lipid rafts in response to LPS was reactive oxygen species (ROS) dependent because it was inhibited by diphenylene iodonium, an inhibitor of NADPH oxidase, and in gp91phox-deficient macrophages. CO selectively inhibited ligand-induced recruitment of TLR4 to lipid rafts, which was also associated with the inhibition of ligand-induced ROS production in macrophages. TLR3 did not translocate to lipid rafts by polyinosine-polycytidylic acid (poly(I:C)). CO had no effect on poly(I:C)-induced ROS production and TLR3 signaling. The inhibitory effect of CO on TLR-induced cytokine production was abolished in gp91phox- deficient macrophages, also indicating a role for NADPH oxidase. CO attenuated LPS-induced NADPH oxidase activity in vitro, potentially by binding to gp91 phox. Thus, CO negatively controlled TLR signaling pathways by inhibiting translocation of TLR to lipid rafts through suppression of NADPH oxidase-dependent ROS generation.

Original languageEnglish
Pages (from-to)2377-2389
Number of pages13
JournalJournal of Experimental Medicine
Volume203
Issue number10
DOIs
Publication statusPublished - 2006
Externally publishedYes

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Toll-Like Receptors
Carbon Monoxide
Reactive Oxygen Species
Lipids
NADPH Oxidase
Macrophages
Poly C
Ligands
Toll-Like Receptor 2
Heme Oxygenase (Decyclizing)
Toll-Like Receptor 4
Heme Oxygenase-1
Heme
Anti-Inflammatory Agents
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Carbon monoxide differentially inhibits TLR signaling pathways by regulating ROS-induced trafficking of TLRs to lipid rafts. / Nakahira, Kiichi; Hong, Pyo Kim; Xue, Hui Geng; Nakao, Atsunori; Wang, Xue; Murase, Noriko; Drain, Peter F.; Wang, Xiaomei; Sasidhar, Madhu; Nabel, Elizabeth G.; Takahashi, Toru; Lukacs, Nicholas W.; Ryter, Stefan W.; Morita, Kiyoshi; Choi, Augustine M K.

In: Journal of Experimental Medicine, Vol. 203, No. 10, 2006, p. 2377-2389.

Research output: Contribution to journalArticle

Nakahira, K, Hong, PK, Xue, HG, Nakao, A, Wang, X, Murase, N, Drain, PF, Wang, X, Sasidhar, M, Nabel, EG, Takahashi, T, Lukacs, NW, Ryter, SW, Morita, K & Choi, AMK 2006, 'Carbon monoxide differentially inhibits TLR signaling pathways by regulating ROS-induced trafficking of TLRs to lipid rafts', Journal of Experimental Medicine, vol. 203, no. 10, pp. 2377-2389. https://doi.org/10.1084/jem.20060845
Nakahira, Kiichi ; Hong, Pyo Kim ; Xue, Hui Geng ; Nakao, Atsunori ; Wang, Xue ; Murase, Noriko ; Drain, Peter F. ; Wang, Xiaomei ; Sasidhar, Madhu ; Nabel, Elizabeth G. ; Takahashi, Toru ; Lukacs, Nicholas W. ; Ryter, Stefan W. ; Morita, Kiyoshi ; Choi, Augustine M K. / Carbon monoxide differentially inhibits TLR signaling pathways by regulating ROS-induced trafficking of TLRs to lipid rafts. In: Journal of Experimental Medicine. 2006 ; Vol. 203, No. 10. pp. 2377-2389.
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