Carbohydrates expressed on Aspergillus fumigatus induce in vivo allergic Th2-type response

Y. Yamashita, M. Okano, Tadashi Yoshino, H. Hattori, T. Yamamoto, T. Watanabe, T. Takishita, T. Akagi, Kazunori Nishizaki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: The mould Aspergillus fumigatus (Af) causes several forms of Th2-biased diseases such as Type I respiratory allergies. This mould contains abundant carbohydrates including glucan, chitin and galactomannan. However, little is known on whether these carbohydrates directly drive Th2 responses in vivo. Objective: We sought to determine the relative role of carbohydrates expressed on crude extracts of Af in the induction of Th2-type antibody production and local eosinophila in mice. Methods: Carbohydrates on native Af were destroyed by sodium metaperiodate under mild conditions. CBA/J mice were sensitized intranasally with native, periodate-treated or mock-treated Af. Histologic changes and production of Af-specific IgE, IgG1 and IgG2a, as well as serum total IgE were determined. Inhibition ELISA for Af-specific IgE was performed using periodate-treated Af as an inhibitor. Results: Mice sensitized with periodate-treated Af displayed significant decreases in both total and specific IgE levels in comparison to mice sensitized with native or mock-treated Af. Furthermore, sensitization and subsequent challenge with periodate-treated Af significantly reduced the degree of eosinophil recruitment into the nasal mucosa, compared to the controls. On the other hand, competitive inhibition showed that periodate-treated Af could inhibit binding between native Af and specific IgE in a similar manner to that of native and mock-treated Af. Conclusion: These results suggest that carbohydrates on Af play a key role as internal adjuvants in inducing the allergic Th2-type response and are not the targets of the induced IgE response.

Original languageEnglish
Pages (from-to)776-782
Number of pages7
JournalClinical and Experimental Allergy
Volume32
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

Aspergillus fumigatus
Carbohydrates
Immunoglobulin E
Fungi
Inbred CBA Mouse
Chitin
Glucans
Nasal Mucosa
Complex Mixtures
Eosinophils
Antibody Formation
metaperiodate

Keywords

  • Allergy
  • Aspergillus fumigatus
  • Eosinophil
  • IgE
  • Mould
  • Mouse
  • Rhinitis
  • Th2

ASJC Scopus subject areas

  • Immunology

Cite this

Carbohydrates expressed on Aspergillus fumigatus induce in vivo allergic Th2-type response. / Yamashita, Y.; Okano, M.; Yoshino, Tadashi; Hattori, H.; Yamamoto, T.; Watanabe, T.; Takishita, T.; Akagi, T.; Nishizaki, Kazunori.

In: Clinical and Experimental Allergy, Vol. 32, No. 5, 2002, p. 776-782.

Research output: Contribution to journalArticle

Yamashita, Y. ; Okano, M. ; Yoshino, Tadashi ; Hattori, H. ; Yamamoto, T. ; Watanabe, T. ; Takishita, T. ; Akagi, T. ; Nishizaki, Kazunori. / Carbohydrates expressed on Aspergillus fumigatus induce in vivo allergic Th2-type response. In: Clinical and Experimental Allergy. 2002 ; Vol. 32, No. 5. pp. 776-782.
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abstract = "Background: The mould Aspergillus fumigatus (Af) causes several forms of Th2-biased diseases such as Type I respiratory allergies. This mould contains abundant carbohydrates including glucan, chitin and galactomannan. However, little is known on whether these carbohydrates directly drive Th2 responses in vivo. Objective: We sought to determine the relative role of carbohydrates expressed on crude extracts of Af in the induction of Th2-type antibody production and local eosinophila in mice. Methods: Carbohydrates on native Af were destroyed by sodium metaperiodate under mild conditions. CBA/J mice were sensitized intranasally with native, periodate-treated or mock-treated Af. Histologic changes and production of Af-specific IgE, IgG1 and IgG2a, as well as serum total IgE were determined. Inhibition ELISA for Af-specific IgE was performed using periodate-treated Af as an inhibitor. Results: Mice sensitized with periodate-treated Af displayed significant decreases in both total and specific IgE levels in comparison to mice sensitized with native or mock-treated Af. Furthermore, sensitization and subsequent challenge with periodate-treated Af significantly reduced the degree of eosinophil recruitment into the nasal mucosa, compared to the controls. On the other hand, competitive inhibition showed that periodate-treated Af could inhibit binding between native Af and specific IgE in a similar manner to that of native and mock-treated Af. Conclusion: These results suggest that carbohydrates on Af play a key role as internal adjuvants in inducing the allergic Th2-type response and are not the targets of the induced IgE response.",
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AU - Okano, M.

AU - Yoshino, Tadashi

AU - Hattori, H.

AU - Yamamoto, T.

AU - Watanabe, T.

AU - Takishita, T.

AU - Akagi, T.

AU - Nishizaki, Kazunori

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AB - Background: The mould Aspergillus fumigatus (Af) causes several forms of Th2-biased diseases such as Type I respiratory allergies. This mould contains abundant carbohydrates including glucan, chitin and galactomannan. However, little is known on whether these carbohydrates directly drive Th2 responses in vivo. Objective: We sought to determine the relative role of carbohydrates expressed on crude extracts of Af in the induction of Th2-type antibody production and local eosinophila in mice. Methods: Carbohydrates on native Af were destroyed by sodium metaperiodate under mild conditions. CBA/J mice were sensitized intranasally with native, periodate-treated or mock-treated Af. Histologic changes and production of Af-specific IgE, IgG1 and IgG2a, as well as serum total IgE were determined. Inhibition ELISA for Af-specific IgE was performed using periodate-treated Af as an inhibitor. Results: Mice sensitized with periodate-treated Af displayed significant decreases in both total and specific IgE levels in comparison to mice sensitized with native or mock-treated Af. Furthermore, sensitization and subsequent challenge with periodate-treated Af significantly reduced the degree of eosinophil recruitment into the nasal mucosa, compared to the controls. On the other hand, competitive inhibition showed that periodate-treated Af could inhibit binding between native Af and specific IgE in a similar manner to that of native and mock-treated Af. Conclusion: These results suggest that carbohydrates on Af play a key role as internal adjuvants in inducing the allergic Th2-type response and are not the targets of the induced IgE response.

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