Carbohydrates expressed on Aspergillus fumigatus induce in vivo allergic Th2-type response

Y. Yamashita, M. Okano, T. Yoshino, H. Hattori, T. Yamamoto, T. Watanabe, T. Takishita, T. Akagi, K. Nishizaki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: The mould Aspergillus fumigatus (Af) causes several forms of Th2-biased diseases such as Type I respiratory allergies. This mould contains abundant carbohydrates including glucan, chitin and galactomannan. However, little is known on whether these carbohydrates directly drive Th2 responses in vivo. Objective: We sought to determine the relative role of carbohydrates expressed on crude extracts of Af in the induction of Th2-type antibody production and local eosinophila in mice. Methods: Carbohydrates on native Af were destroyed by sodium metaperiodate under mild conditions. CBA/J mice were sensitized intranasally with native, periodate-treated or mock-treated Af. Histologic changes and production of Af-specific IgE, IgG1 and IgG2a, as well as serum total IgE were determined. Inhibition ELISA for Af-specific IgE was performed using periodate-treated Af as an inhibitor. Results: Mice sensitized with periodate-treated Af displayed significant decreases in both total and specific IgE levels in comparison to mice sensitized with native or mock-treated Af. Furthermore, sensitization and subsequent challenge with periodate-treated Af significantly reduced the degree of eosinophil recruitment into the nasal mucosa, compared to the controls. On the other hand, competitive inhibition showed that periodate-treated Af could inhibit binding between native Af and specific IgE in a similar manner to that of native and mock-treated Af. Conclusion: These results suggest that carbohydrates on Af play a key role as internal adjuvants in inducing the allergic Th2-type response and are not the targets of the induced IgE response.

Original languageEnglish
Pages (from-to)776-782
Number of pages7
JournalClinical and Experimental Allergy
Volume32
Issue number5
DOIs
Publication statusPublished - May 30 2002

Keywords

  • Allergy
  • Aspergillus fumigatus
  • Eosinophil
  • IgE
  • Mould
  • Mouse
  • Rhinitis
  • Th2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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