Capsid structure of dsRNA fungal viruses

Daniel Luque, Carlos P. Mata, Nobuhiro Suzuki, Said A. Ghabrial, José R. Castón

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). These capsids show structural variations of the same framework, with 60 asymmetric or symmetric homodimers for ScV-L-A and PsV-F, respectively, monomers with a duplicated similar domain for PcV, and heterodimers of two different proteins for RnQV1. Mycovirus capsid proteins (CP) share a conserved α-helical domain, although the latter may carry different peptides inserted at preferential hotspots. Insertions in the CP outer surface are likely associated with enzymatic activities. Within the capsid, fungal dsRNA viruses show a low degree of genome compaction compared to reoviruses, and contain one to two copies of the RNA-polymerase complex per virion.

Original languageEnglish
Article number481
JournalViruses
Volume10
Issue number9
DOIs
Publication statusPublished - Sep 7 2018

Fingerprint

Capsid
Viruses
Penicillium chrysogenum
Penicillium
Capsid Proteins
DNA-Directed RNA Polymerases
Double-Stranded RNA
Viral Genome
RNA Viruses
Life Cycle Stages
Virion
Saccharomyces cerevisiae
Fungal Viruses
Genome
Peptides
Proteins

Keywords

  • Capsid protein
  • Capsid structure
  • dsRNA virus
  • Mycovirus
  • PcV
  • PsV-F
  • RnQV1
  • ScV-L-A
  • Viral lineage
  • Virus evolution

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Luque, D., Mata, C. P., Suzuki, N., Ghabrial, S. A., & Castón, J. R. (2018). Capsid structure of dsRNA fungal viruses. Viruses, 10(9), [481]. https://doi.org/10.3390/v10090481

Capsid structure of dsRNA fungal viruses. / Luque, Daniel; Mata, Carlos P.; Suzuki, Nobuhiro; Ghabrial, Said A.; Castón, José R.

In: Viruses, Vol. 10, No. 9, 481, 07.09.2018.

Research output: Contribution to journalReview article

Luque, D, Mata, CP, Suzuki, N, Ghabrial, SA & Castón, JR 2018, 'Capsid structure of dsRNA fungal viruses', Viruses, vol. 10, no. 9, 481. https://doi.org/10.3390/v10090481
Luque D, Mata CP, Suzuki N, Ghabrial SA, Castón JR. Capsid structure of dsRNA fungal viruses. Viruses. 2018 Sep 7;10(9). 481. https://doi.org/10.3390/v10090481
Luque, Daniel ; Mata, Carlos P. ; Suzuki, Nobuhiro ; Ghabrial, Said A. ; Castón, José R. / Capsid structure of dsRNA fungal viruses. In: Viruses. 2018 ; Vol. 10, No. 9.
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