Capillary electrophoresis with laser-induced fluorescence detection for application in intracellular investigation of anthracyclines and multidrug resistance proteins

Julius Mbuna, Takashi Kaneta

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) is a powerful method for the trace analysis of cellular components. This review presents a summary of topics for the direct analysis of anthracyclines and multidrug resistance proteins in cancerous cells. A micellar electrokinetic chromatography (MEKC) method that does not use organic solvents, and hence prevents the precipitation of proteins in cellular samples, was shown to be a reliable method for the determination of several anthracyclines including the epimeric doxorubicin and epirubicin. A fast CE-based immunoassay for investigating transporter multidrug resistance associated protein (MRP1) was also developed for routine or explorative analysis of the levels of transporter proteins in cancerous cells. A combination of the developed MEKCLIF method and the CE immunoassay (CEIA) method has permitted the analysis of anthracyclines and MRP1 in a cell line to show the relationship between the levels of MRP1 and amount of anthracyclines in cancerous cells.

Original languageEnglish
Pages (from-to)1121-1128
Number of pages8
JournalAnalytical Sciences
Volume31
Issue number11
DOIs
Publication statusPublished - 2015

Fingerprint

P-Glycoproteins
Capillary electrophoresis
Anthracyclines
Fluorescence
Lasers
Trace analysis
Multidrug Resistance-Associated Proteins
Epirubicin
Chromatography
Doxorubicin
Organic solvents
Proteins
Cells

Keywords

  • Anthracycline
  • Cancer cell
  • Capillary electrophoresis
  • Capillary electrophoresis immunoassay
  • Laser-induced fluorescence
  • Micellar electrokinetic chromatography
  • Multidrug resistance associated protein

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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abstract = "Capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) is a powerful method for the trace analysis of cellular components. This review presents a summary of topics for the direct analysis of anthracyclines and multidrug resistance proteins in cancerous cells. A micellar electrokinetic chromatography (MEKC) method that does not use organic solvents, and hence prevents the precipitation of proteins in cellular samples, was shown to be a reliable method for the determination of several anthracyclines including the epimeric doxorubicin and epirubicin. A fast CE-based immunoassay for investigating transporter multidrug resistance associated protein (MRP1) was also developed for routine or explorative analysis of the levels of transporter proteins in cancerous cells. A combination of the developed MEKCLIF method and the CE immunoassay (CEIA) method has permitted the analysis of anthracyclines and MRP1 in a cell line to show the relationship between the levels of MRP1 and amount of anthracyclines in cancerous cells.",
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