Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb

Yasuhiro Takahara, Masuo Senda, Hiroyuki Hashizume, Yukihisa Yagata, Hajime Inoue

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We observed differences in the capillary architecture of the skeletal muscles that have different fiber metabolism. The soleus, the vastus intermedius and the tibialis anterior muscles of adult Wistar rats were prepared using two different techniques. Samples for adenosine triphosphatase (ATPase) staining were prepared following Dubovitz's method, and the distributions of fiber type, Types 1, 2A and 2B, were analyzed. Then, corrosion casts of capillary architecture of these muscles prepared following Murakami's method were observed with a scanning electron microscope (SEM) and compared with the fiber distribution. The fiber type composition of the soleus muscle showed Type 1 (slow-twitch) dominance and that of the vastus intermedius and the tibialis anterior muscle showed Type 2 (fast-twitch) dominance. The capillaries of the soleus muscle were tortuous, and this was thought to be advantageous for blood supply. In contrast, the capillaries of the vastus intermedius and tibialis anterior muscles had a relatively parallel pattern. Additionally, two different patterns of capillary architecture that appeared to correspond to certain metabolic characteristic of different muscle fiber types were preserved with corrosion casting. In conclusion, comparative studies on capillary architecture of the skeletal muscles are useful for analyses of its function.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalActa Medica Okayama
Volume50
Issue number4
Publication statusPublished - Aug 1996

Fingerprint

Muscle
Rats
Skeletal Muscle
Extremities
Quadriceps Muscle
Muscles
Fibers
Corrosion Casting
Corrosion
Adenosine Triphosphatases
Wistar Rats
Electrons
Staining and Labeling
Metabolism
Casting
Blood
Electron microscopes
Scanning
Chemical analysis

Keywords

  • ATPase staining
  • Capillary architecture
  • Corrosion cast
  • Muscle fiber type
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Surgery

Cite this

Takahara, Y., Senda, M., Hashizume, H., Yagata, Y., & Inoue, H. (1996). Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb. Acta Medica Okayama, 50(4), 211-218.

Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb. / Takahara, Yasuhiro; Senda, Masuo; Hashizume, Hiroyuki; Yagata, Yukihisa; Inoue, Hajime.

In: Acta Medica Okayama, Vol. 50, No. 4, 08.1996, p. 211-218.

Research output: Contribution to journalArticle

Takahara, Y, Senda, M, Hashizume, H, Yagata, Y & Inoue, H 1996, 'Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb', Acta Medica Okayama, vol. 50, no. 4, pp. 211-218.
Takahara Y, Senda M, Hashizume H, Yagata Y, Inoue H. Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb. Acta Medica Okayama. 1996 Aug;50(4):211-218.
Takahara, Yasuhiro ; Senda, Masuo ; Hashizume, Hiroyuki ; Yagata, Yukihisa ; Inoue, Hajime. / Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb. In: Acta Medica Okayama. 1996 ; Vol. 50, No. 4. pp. 211-218.
@article{eccd446e710d4e6ebfc76d59c2b4ed4c,
title = "Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb",
abstract = "We observed differences in the capillary architecture of the skeletal muscles that have different fiber metabolism. The soleus, the vastus intermedius and the tibialis anterior muscles of adult Wistar rats were prepared using two different techniques. Samples for adenosine triphosphatase (ATPase) staining were prepared following Dubovitz's method, and the distributions of fiber type, Types 1, 2A and 2B, were analyzed. Then, corrosion casts of capillary architecture of these muscles prepared following Murakami's method were observed with a scanning electron microscope (SEM) and compared with the fiber distribution. The fiber type composition of the soleus muscle showed Type 1 (slow-twitch) dominance and that of the vastus intermedius and the tibialis anterior muscle showed Type 2 (fast-twitch) dominance. The capillaries of the soleus muscle were tortuous, and this was thought to be advantageous for blood supply. In contrast, the capillaries of the vastus intermedius and tibialis anterior muscles had a relatively parallel pattern. Additionally, two different patterns of capillary architecture that appeared to correspond to certain metabolic characteristic of different muscle fiber types were preserved with corrosion casting. In conclusion, comparative studies on capillary architecture of the skeletal muscles are useful for analyses of its function.",
keywords = "ATPase staining, Capillary architecture, Corrosion cast, Muscle fiber type, Skeletal muscle",
author = "Yasuhiro Takahara and Masuo Senda and Hiroyuki Hashizume and Yukihisa Yagata and Hajime Inoue",
year = "1996",
month = "8",
language = "English",
volume = "50",
pages = "211--218",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "4",

}

TY - JOUR

T1 - Capillary Architecture in the Skeletal Muscles in the Rat Hind Limb

AU - Takahara, Yasuhiro

AU - Senda, Masuo

AU - Hashizume, Hiroyuki

AU - Yagata, Yukihisa

AU - Inoue, Hajime

PY - 1996/8

Y1 - 1996/8

N2 - We observed differences in the capillary architecture of the skeletal muscles that have different fiber metabolism. The soleus, the vastus intermedius and the tibialis anterior muscles of adult Wistar rats were prepared using two different techniques. Samples for adenosine triphosphatase (ATPase) staining were prepared following Dubovitz's method, and the distributions of fiber type, Types 1, 2A and 2B, were analyzed. Then, corrosion casts of capillary architecture of these muscles prepared following Murakami's method were observed with a scanning electron microscope (SEM) and compared with the fiber distribution. The fiber type composition of the soleus muscle showed Type 1 (slow-twitch) dominance and that of the vastus intermedius and the tibialis anterior muscle showed Type 2 (fast-twitch) dominance. The capillaries of the soleus muscle were tortuous, and this was thought to be advantageous for blood supply. In contrast, the capillaries of the vastus intermedius and tibialis anterior muscles had a relatively parallel pattern. Additionally, two different patterns of capillary architecture that appeared to correspond to certain metabolic characteristic of different muscle fiber types were preserved with corrosion casting. In conclusion, comparative studies on capillary architecture of the skeletal muscles are useful for analyses of its function.

AB - We observed differences in the capillary architecture of the skeletal muscles that have different fiber metabolism. The soleus, the vastus intermedius and the tibialis anterior muscles of adult Wistar rats were prepared using two different techniques. Samples for adenosine triphosphatase (ATPase) staining were prepared following Dubovitz's method, and the distributions of fiber type, Types 1, 2A and 2B, were analyzed. Then, corrosion casts of capillary architecture of these muscles prepared following Murakami's method were observed with a scanning electron microscope (SEM) and compared with the fiber distribution. The fiber type composition of the soleus muscle showed Type 1 (slow-twitch) dominance and that of the vastus intermedius and the tibialis anterior muscle showed Type 2 (fast-twitch) dominance. The capillaries of the soleus muscle were tortuous, and this was thought to be advantageous for blood supply. In contrast, the capillaries of the vastus intermedius and tibialis anterior muscles had a relatively parallel pattern. Additionally, two different patterns of capillary architecture that appeared to correspond to certain metabolic characteristic of different muscle fiber types were preserved with corrosion casting. In conclusion, comparative studies on capillary architecture of the skeletal muscles are useful for analyses of its function.

KW - ATPase staining

KW - Capillary architecture

KW - Corrosion cast

KW - Muscle fiber type

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=0030202968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030202968&partnerID=8YFLogxK

M3 - Article

C2 - 8874583

AN - SCOPUS:0030202968

VL - 50

SP - 211

EP - 218

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 4

ER -