Cancer-associated stromal cells promote the contribution of mmp2-positive bone marrow-derived cells to oral squamous cell carcinoma invasion

May Wathone Oo, Hotaka Kawai, Kiyofumi Takabatake, Qiusheng Shan, Htoo Shwe Eain, Shintaro Sukegawa, Keisuke Nakano, Hitoshi Nagatsuka

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Tumor stromal components contribute to tumor development and invasion. However, the role of stromal cells in the contribution of bone marrow-derived cells (BMDCs) in oral squamous cell carcinoma (OSCC) invasion is unclear. In the present study, we created two different invasive OSCC patient-derived stroma xenografts (PDSXs) and analyzed and compared the effects of stromal cells on the relation of BMDCs and tumor invasion. We isolated stromal cells from two OSCC patients: less invasive verrucous OSCC (VSCC) and highly invasive conventional OSCC (SCC) and co-xenografted with the OSCC cell line (HSC-2) on green fluorescent protein (GFP)-positive bone marrow (BM) cells transplanted mice. We traced the GFP-positive BM cells by immunohistochemistry (IHC) and detected matrix metalloproteinase 2 (MMP2) expression on BM cells by double fluorescent IHC. The results indicated that the SCC-PDSX promotes MMP2-positive BMDCs recruitment to the invasive front line of the tumor. Furthermore, microarray analysis revealed that the expressions of interleukin 6; IL-6 mRNA and interleukin 1 beta; IL1B mRNA were higher in SCC stromal cells than in VSCC stromal cells. Thus, our study first reports that IL-6 and IL1B might be the potential stromal factors promoting the contribution of MMP2-positive BMDCs to OSCC invasion.

Original languageEnglish
Article number137
JournalCancers
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 1 2022

Keywords

  • Bone marrow-derived cells (BMDCs)
  • MMP2
  • Oral squamous cell carcinoma invasion
  • Patient-derived stromal cell xenograft (PDSX)
  • Stromal factor IL-6
  • Stromal factor IL1B

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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