Camptothecin induces urokinase-type plasminogen activator gene-expression in human RC-K8 malignant lymphoma and H69 small cell lung cancer cells

Misako Shibakura, Kenji Niiya, Toru Kiguchi, Yasunari Nakata, Mitsune Tanimoto

Research output: Contribution to journalArticle

Abstract

We previously reported that anthracyclines, which could generate reactive oxygen species (ROS), could induce the urokinase-type plasminogen activator (uPA) gene expression in human RC-K8 malignant lymphoma cells and in H69 small cell lung cancer (SCLC) cells. In screening other uPA-inducible anti-cancer agents, we found that camptothecin (CPT) and its derivative, SN38, could induce uPA in RC-K8 and H69 cells. CPT and SN38, which are also used for the treatment of lymphoma and SCLC, significantly increased the uPA accumulation in the conditioned media of both cells in a dose-dependent manner. The maximum induction of uPA mRNA levels was observed 24 h after stimulation. Pretreatment with pyrrolidine dithiocarbamate (PDTC), an anti-oxidant, inhibited the CPT-induced uPA mRNA expression. Thus, CPT induces uPA through gene expression, and, therefore, CPT may influence the tumor-cell biology by up-regulating the uPA/plasmin system.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalActa medica Okayama
Volume56
Issue number5
Publication statusPublished - 2002

Keywords

  • CPT
  • H69
  • RC-K8
  • SN38
  • uPA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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