Calcium specificity signaling mechanisms in abscisic acid signal transduction in arabidopsis guard cells

Benjamin Brandt, Shintaro Munemasa, Cun Wang, Desiree Nguyen, Taiming Yong, Paul G. Yang, Elly Poretsky, Thomas F. Belknap, Rainer Waadt, Fernando Alemań, Julian I. Schroeder

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92 Citations (Scopus)

Abstract

A central question is how specificity in cellular responses to the eukaryotic second messenger Ca2+ is achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system for in depth investigation of Ca2+-signaling specificity in plants. In intact guard cells, abscisic acid (ABA) enhances (primes) the Ca2+-sensitivity of downstream signaling events that result in activation of S-type anion channels during stomatal closure, providing a specificity mechanism in Ca2+-signaling. However, the underlying genetic and biochemical mechanisms remain unknown. Here we show impairment of ABA signal transduction in stomata of calcium-dependent protein kinase quadruple mutant plants. Interestingly, protein phosphatase 2Cs prevent non-specific Ca2+-signaling. Moreover, we demonstrate an unexpected interdependence of the Ca2+-dependent and Ca2+-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at two key phosphorylation sites in SLAC1. We identify novel mechanisms ensuring specificity and robustness within stomatal Ca2+-signaling on a cellular, genetic, and biochemical level.

Original languageEnglish
Article numbere03599
Pages (from-to)1-25
Number of pages25
JournaleLife
Volume4
Issue numberJULY 2015
DOIs
Publication statusPublished - Jun 20 2015

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Brandt, B., Munemasa, S., Wang, C., Nguyen, D., Yong, T., Yang, P. G., Poretsky, E., Belknap, T. F., Waadt, R., Alemań, F., & Schroeder, J. I. (2015). Calcium specificity signaling mechanisms in abscisic acid signal transduction in arabidopsis guard cells. eLife, 4(JULY 2015), 1-25. [e03599]. https://doi.org/10.7554/eLife.03599