Calcium ion triggers rapid morphological oscillation of chloride cells in the mudskipper, Periophthalmus modestus

T. Sakamoto, M. Ando

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Euryhaline teleosts rapidly regulate their ion flux at the chloride cells on entry to different salinities. The external trigger(s) for the rapid opening and closing of the chloride cell apical surface, the site of salt secretion, were examined in the skin of the mudskipper. With DASPEI (a mitochondrial probe) and Concanavalin-A (an apical surface marker of chloride cells), chloride cells were classified into two groups: those in contact and those not in contact with the external water. The fraction of chloride cells in contact with the water increased dramatically 1.5 h after transfer to seawater, and similarly after transfer to 10 mmol·l-1 CaCl2 solution. In comparison, transfer to 1.1 mol·l-1 mannitol, 0.5 mol·l-1 NaCl or 50 mmol·l-1 MgCl2 resulted in increases of only 40-60% of those in the seawater-transfer group. After return of the fish from 10 mmol·l-1 CaCl2 to freshwater, the cells in contact with the water decreased. Environmental Ca2+ is the trigger for the morphological oscillation of chloride cell apical surface, presumably through modifications in Ca2+ flux.

Original languageEnglish
Pages (from-to)435-439
Number of pages5
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume172
Issue number5
DOIs
Publication statusPublished - Dec 1 2002
Externally publishedYes

Keywords

  • Calcium ion
  • Chloride cell
  • Mudskipper
  • Osmoregulation
  • Salinity adaptation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Physiology
  • Animal Science and Zoology
  • Endocrinology

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