Calcium ion exchange in crystalline gelsolin

Sakesit Chumnarnsilpa, Anantasak Loonchanta, Bo Xue, Han Choe, Dunja Urosev, Hui Wang, Uno Lindberg, Leslie D. Burtnick, Robert C. Robinson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Gelsolin is a calcium and pH-sensitive modulator of actin filament length. Here, we use X-ray crystallography to examine the extraction and exchange of calcium ions from their binding sites in different crystalline forms of the activated N and C-terminal halves of gelsolin, G1-G3 and G4-G6, respectively. We demonstrate that the combination of calcium and low pH activating conditions do not induce conformational changes in G4-G6 beyond those elicited by calcium alone. EGTA is able to remove calcium ions bound to the type I and type II metal ion-binding sites in G4-G6. Constrained by crystal contacts and stabilized by interdomain interaction surfaces, the gross structure of calcium-depleted G4-G6 remains that of the activated form. However, high-resolution details of changes in the ion-binding sites may represent the initial steps toward restoration of the arrangement of domains found in the calcium-free inactive form of gelsolin in solution. Furthermore, bathing crystals with the trivalent calcium ion mimic, Tb3+, results in anomalous scattering data that permit unequivocal localization of terbium ions in each of the proposed type I and type II ion-binding sites of both halves of gelsolin. In contrast to predictions based on solution studies, we find that no calcium ion is immune to exchange.

Original languageEnglish
Pages (from-to)773-782
Number of pages10
JournalJournal of Molecular Biology
Volume357
Issue number3
DOIs
Publication statusPublished - Mar 31 2006
Externally publishedYes

Fingerprint

Gelsolin
Ion Exchange
Calcium
Ions
Binding Sites
Terbium
Egtazic Acid
X Ray Crystallography
Actin Cytoskeleton
Metals

Keywords

  • Actin
  • Calcium activation
  • Conformational change
  • Gelsolin
  • Protein crystallography

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Chumnarnsilpa, S., Loonchanta, A., Xue, B., Choe, H., Urosev, D., Wang, H., ... Robinson, R. C. (2006). Calcium ion exchange in crystalline gelsolin. Journal of Molecular Biology, 357(3), 773-782. https://doi.org/10.1016/j.jmb.2006.01.026

Calcium ion exchange in crystalline gelsolin. / Chumnarnsilpa, Sakesit; Loonchanta, Anantasak; Xue, Bo; Choe, Han; Urosev, Dunja; Wang, Hui; Lindberg, Uno; Burtnick, Leslie D.; Robinson, Robert C.

In: Journal of Molecular Biology, Vol. 357, No. 3, 31.03.2006, p. 773-782.

Research output: Contribution to journalArticle

Chumnarnsilpa, S, Loonchanta, A, Xue, B, Choe, H, Urosev, D, Wang, H, Lindberg, U, Burtnick, LD & Robinson, RC 2006, 'Calcium ion exchange in crystalline gelsolin', Journal of Molecular Biology, vol. 357, no. 3, pp. 773-782. https://doi.org/10.1016/j.jmb.2006.01.026
Chumnarnsilpa S, Loonchanta A, Xue B, Choe H, Urosev D, Wang H et al. Calcium ion exchange in crystalline gelsolin. Journal of Molecular Biology. 2006 Mar 31;357(3):773-782. https://doi.org/10.1016/j.jmb.2006.01.026
Chumnarnsilpa, Sakesit ; Loonchanta, Anantasak ; Xue, Bo ; Choe, Han ; Urosev, Dunja ; Wang, Hui ; Lindberg, Uno ; Burtnick, Leslie D. ; Robinson, Robert C. / Calcium ion exchange in crystalline gelsolin. In: Journal of Molecular Biology. 2006 ; Vol. 357, No. 3. pp. 773-782.
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