Calcium hydroxide suppresses Porphyromonas endodontalis lipopolysaccharide-induced bone destruction

J. Guo, D. Yang, H. Okamura, J. Teramachi, K. Ochiai, L. Qiu, T. Haneji

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Porphyromonas endodontalis and its main virulence factor, lipopolysaccharide (LPS), are associated with the development of periapical diseases and alveolar bone loss. Calcium hydroxide is commonly used for endodontic therapy. However, the effects of calcium hydroxide on the virulence of P. endodontalis LPS and the mechanism of P. endodontalis LPS-induced bone destruction are not clear. Calcium hydroxide rescued the P. endodontalis LPS-suppressed viability of MC3T3-E1 cells and activity of nuclear factor-κB (NF-κB) in these cells, resulting in the reduced expression of interleukin-6 and tumor necrosis factor-α. In addition, calcium hydroxide inhibited P. endodontalis LPS-induced osteoclastogenesis by decreasing the activities of NF-κB, p38, and ERK1/2 and the expression of nuclear factor of activated T-cell cytoplasmic 1 in RAW264.7 cells. Calcium hydroxide also rescued the P. endodontalis LPS-induced osteoclastogenesis and bone destruction in mouse calvaria. Taken together, our present results indicate that calcium hydroxide suppressed bone destruction by attenuating the virulence of P. endodontalis LPS on bone cells.

Original languageEnglish
Pages (from-to)508-513
Number of pages6
JournalJournal of dental research
Volume93
Issue number5
DOIs
Publication statusPublished - May 2014
Externally publishedYes

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Keywords

  • bone resorption
  • interleukin-6
  • nuclear factor of activated T-cell cytoplasmic 1
  • osteoblasts
  • osteoclasts
  • tumor necrosis factor-α

ASJC Scopus subject areas

  • Dentistry(all)

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