Calcium content of oxygen-evolving Photosystem II preparations from higher plants. Effects of NaCl treatment

Jian-Ren Shen, Kazuhiko Satoh, Sakae Katoh

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The abundance of bound Ca2+ in four Photosystem II oxygen-evolving preparations from spinach and rice were determined after removal of contaminating Ca2+ with chelex 100 (Kashino, Y., Satoh, K. and Katoh, S. (1986) FEBS Lett. 205, 150-154). (1) Spinach membrane preparations which evolved oxygen at rates of 350-500 μmol per mg chlorophyll (Chl) per h contained, on average, 1.8 Ca2+ per 200 Chl, whereas a mean value of 2.1 Ca2+ per 200 Chl was obtained with more active spinach preparations, showing oxygen-evolving rates of 500-800 μmol per mg Chl per h. The most active membrane preparations, which evolved oxygen at rates of 750-1050 μmol per mg Chl per h, were isolated from rice seedlings. The rice preparations also contained 2.1 Ca2+ per 200 Chl. Thus, different activities of the three preparations cannot be related to difference in their Ca2+ abundance and the maximum number of Ca2+ needed to promote high rates of oxygen evolution is 2 Ca2+ per 200 Chl in higher plants. (2) Highly purified spinach oxygen-evolving complexes (Ikeuchi, M., Yuasa, M. and Inoue, Y. (1985) FEBS Lett. 185, 316-323) contained 1 Ca2+ per 50 Chl. (3) Treatment of the spinach membrane preparations with high concentrations of NaCl, either in the dark or light, caused no significant loss of the bound Ca2+, although the treatment strongly inhibited oxygen evolution and the inhibition was considerably reversed by addition of 5 mM CaCl2. Exposure of the NaCl-washed membranes to EGTA failed to reduce the amount of bound Ca2+. Thus, inactivation of oxygen evolution by the salt wash is not related to release of Ca2+. (4) The abundance of Ca2+ in spinach membrane preparations was not, or only slightly, affected by treatments with 1 mM NH2OH, 1 M MgCl2 or 0.8 M Tris, which solubilized either Mn or the three extrinsic proteins associated with the membranes, or both. It is concluded that the three proteins are not involved in the Ca2+ binding. The number of Ca2+ functioning in Photosystem II electron transport of higher plants is discussed.

Original languageEnglish
Pages (from-to)358-364
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume933
Issue number2
DOIs
Publication statusPublished - Apr 22 1988
Externally publishedYes

Fingerprint

Plant Preparations
Photosystem II Protein Complex
Chlorophyll
Spinacia oleracea
Oxygen
Calcium
Membranes
Magnesium Chloride
Egtazic Acid
Electron Transport
Seedlings
Membrane Proteins
Proteins
Salts
Light

Keywords

  • Calcium
  • NaCl treatment, 23 kDa-
  • Oxygen evolution
  • Photosystem II
  • Protein

ASJC Scopus subject areas

  • Biophysics

Cite this

Calcium content of oxygen-evolving Photosystem II preparations from higher plants. Effects of NaCl treatment. / Shen, Jian-Ren; Satoh, Kazuhiko; Katoh, Sakae.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 933, No. 2, 22.04.1988, p. 358-364.

Research output: Contribution to journalArticle

@article{3d3c625088a94f4a9c56510851055fdb,
title = "Calcium content of oxygen-evolving Photosystem II preparations from higher plants. Effects of NaCl treatment",
abstract = "The abundance of bound Ca2+ in four Photosystem II oxygen-evolving preparations from spinach and rice were determined after removal of contaminating Ca2+ with chelex 100 (Kashino, Y., Satoh, K. and Katoh, S. (1986) FEBS Lett. 205, 150-154). (1) Spinach membrane preparations which evolved oxygen at rates of 350-500 μmol per mg chlorophyll (Chl) per h contained, on average, 1.8 Ca2+ per 200 Chl, whereas a mean value of 2.1 Ca2+ per 200 Chl was obtained with more active spinach preparations, showing oxygen-evolving rates of 500-800 μmol per mg Chl per h. The most active membrane preparations, which evolved oxygen at rates of 750-1050 μmol per mg Chl per h, were isolated from rice seedlings. The rice preparations also contained 2.1 Ca2+ per 200 Chl. Thus, different activities of the three preparations cannot be related to difference in their Ca2+ abundance and the maximum number of Ca2+ needed to promote high rates of oxygen evolution is 2 Ca2+ per 200 Chl in higher plants. (2) Highly purified spinach oxygen-evolving complexes (Ikeuchi, M., Yuasa, M. and Inoue, Y. (1985) FEBS Lett. 185, 316-323) contained 1 Ca2+ per 50 Chl. (3) Treatment of the spinach membrane preparations with high concentrations of NaCl, either in the dark or light, caused no significant loss of the bound Ca2+, although the treatment strongly inhibited oxygen evolution and the inhibition was considerably reversed by addition of 5 mM CaCl2. Exposure of the NaCl-washed membranes to EGTA failed to reduce the amount of bound Ca2+. Thus, inactivation of oxygen evolution by the salt wash is not related to release of Ca2+. (4) The abundance of Ca2+ in spinach membrane preparations was not, or only slightly, affected by treatments with 1 mM NH2OH, 1 M MgCl2 or 0.8 M Tris, which solubilized either Mn or the three extrinsic proteins associated with the membranes, or both. It is concluded that the three proteins are not involved in the Ca2+ binding. The number of Ca2+ functioning in Photosystem II electron transport of higher plants is discussed.",
keywords = "Calcium, NaCl treatment, 23 kDa-, Oxygen evolution, Photosystem II, Protein",
author = "Jian-Ren Shen and Kazuhiko Satoh and Sakae Katoh",
year = "1988",
month = "4",
day = "22",
doi = "10.1016/0005-2728(88)90043-6",
language = "English",
volume = "933",
pages = "358--364",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Calcium content of oxygen-evolving Photosystem II preparations from higher plants. Effects of NaCl treatment

AU - Shen, Jian-Ren

AU - Satoh, Kazuhiko

AU - Katoh, Sakae

PY - 1988/4/22

Y1 - 1988/4/22

N2 - The abundance of bound Ca2+ in four Photosystem II oxygen-evolving preparations from spinach and rice were determined after removal of contaminating Ca2+ with chelex 100 (Kashino, Y., Satoh, K. and Katoh, S. (1986) FEBS Lett. 205, 150-154). (1) Spinach membrane preparations which evolved oxygen at rates of 350-500 μmol per mg chlorophyll (Chl) per h contained, on average, 1.8 Ca2+ per 200 Chl, whereas a mean value of 2.1 Ca2+ per 200 Chl was obtained with more active spinach preparations, showing oxygen-evolving rates of 500-800 μmol per mg Chl per h. The most active membrane preparations, which evolved oxygen at rates of 750-1050 μmol per mg Chl per h, were isolated from rice seedlings. The rice preparations also contained 2.1 Ca2+ per 200 Chl. Thus, different activities of the three preparations cannot be related to difference in their Ca2+ abundance and the maximum number of Ca2+ needed to promote high rates of oxygen evolution is 2 Ca2+ per 200 Chl in higher plants. (2) Highly purified spinach oxygen-evolving complexes (Ikeuchi, M., Yuasa, M. and Inoue, Y. (1985) FEBS Lett. 185, 316-323) contained 1 Ca2+ per 50 Chl. (3) Treatment of the spinach membrane preparations with high concentrations of NaCl, either in the dark or light, caused no significant loss of the bound Ca2+, although the treatment strongly inhibited oxygen evolution and the inhibition was considerably reversed by addition of 5 mM CaCl2. Exposure of the NaCl-washed membranes to EGTA failed to reduce the amount of bound Ca2+. Thus, inactivation of oxygen evolution by the salt wash is not related to release of Ca2+. (4) The abundance of Ca2+ in spinach membrane preparations was not, or only slightly, affected by treatments with 1 mM NH2OH, 1 M MgCl2 or 0.8 M Tris, which solubilized either Mn or the three extrinsic proteins associated with the membranes, or both. It is concluded that the three proteins are not involved in the Ca2+ binding. The number of Ca2+ functioning in Photosystem II electron transport of higher plants is discussed.

AB - The abundance of bound Ca2+ in four Photosystem II oxygen-evolving preparations from spinach and rice were determined after removal of contaminating Ca2+ with chelex 100 (Kashino, Y., Satoh, K. and Katoh, S. (1986) FEBS Lett. 205, 150-154). (1) Spinach membrane preparations which evolved oxygen at rates of 350-500 μmol per mg chlorophyll (Chl) per h contained, on average, 1.8 Ca2+ per 200 Chl, whereas a mean value of 2.1 Ca2+ per 200 Chl was obtained with more active spinach preparations, showing oxygen-evolving rates of 500-800 μmol per mg Chl per h. The most active membrane preparations, which evolved oxygen at rates of 750-1050 μmol per mg Chl per h, were isolated from rice seedlings. The rice preparations also contained 2.1 Ca2+ per 200 Chl. Thus, different activities of the three preparations cannot be related to difference in their Ca2+ abundance and the maximum number of Ca2+ needed to promote high rates of oxygen evolution is 2 Ca2+ per 200 Chl in higher plants. (2) Highly purified spinach oxygen-evolving complexes (Ikeuchi, M., Yuasa, M. and Inoue, Y. (1985) FEBS Lett. 185, 316-323) contained 1 Ca2+ per 50 Chl. (3) Treatment of the spinach membrane preparations with high concentrations of NaCl, either in the dark or light, caused no significant loss of the bound Ca2+, although the treatment strongly inhibited oxygen evolution and the inhibition was considerably reversed by addition of 5 mM CaCl2. Exposure of the NaCl-washed membranes to EGTA failed to reduce the amount of bound Ca2+. Thus, inactivation of oxygen evolution by the salt wash is not related to release of Ca2+. (4) The abundance of Ca2+ in spinach membrane preparations was not, or only slightly, affected by treatments with 1 mM NH2OH, 1 M MgCl2 or 0.8 M Tris, which solubilized either Mn or the three extrinsic proteins associated with the membranes, or both. It is concluded that the three proteins are not involved in the Ca2+ binding. The number of Ca2+ functioning in Photosystem II electron transport of higher plants is discussed.

KW - Calcium

KW - NaCl treatment, 23 kDa-

KW - Oxygen evolution

KW - Photosystem II

KW - Protein

UR - http://www.scopus.com/inward/record.url?scp=0001167854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001167854&partnerID=8YFLogxK

U2 - 10.1016/0005-2728(88)90043-6

DO - 10.1016/0005-2728(88)90043-6

M3 - Article

AN - SCOPUS:0001167854

VL - 933

SP - 358

EP - 364

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 2

ER -