Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells

Zhen Ming Pel, Yoshiyuki Murata, Gregor Benning, Sébastien Thomine, Birgit Klüsener, Gethyn J. Allen, Erwin Grill, Julian I. Schroeder

Research output: Contribution to journalArticle

1372 Citations (Scopus)

Abstract

Drought is a major threat to agricultural production. Plants synthesize the hormone abscisic acid (ABA) in response to drought, triggering a signalling cascade in guard cells that results in stomatal closure, thus reducing water loss. ABA triggers an increase in cytosolic calcium in guard cells ([Ca2+](cyt)) that has been proposed to include Ca2+ influx across the plasma membrane. However, direct recordings of Ca2+ currents have been limited and the upstream activation mechanisms of plasma membrane Ca2+ channels remain unknown. Here we report activation of Ca2+-permeable channels in the plasma membrane of Arabidopsis guard cells by hydrogen peroxide. The H2O2-activated Ca2+ channels mediate both influx of Ca2+ in protoplasts and increases in [Ca2+](cyt) in intact guard cells. ABA induces the production of H2O2 in guard cells. If H2O2 production is blocked, ABA-induced closure of stomata is inhibited. Moreover, activation of Ca2+ channels by H2O2 and ABA- and H2O2-induced stomatal closing are disrupted in the recessive ABA-insensitive mutant gca2. These data indicate that ABA-induced H2O2 production and the H2O2-activated Ca2+ channels are important mechanisms for ABA-induced stomatal closing.

Original languageEnglish
Pages (from-to)731-734
Number of pages4
JournalNature
Volume406
Issue number6797
DOIs
Publication statusPublished - Aug 17 2000
Externally publishedYes

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Abscisic Acid
Calcium Channels
Hydrogen Peroxide
Droughts
Cell Membrane
Plant Growth Regulators
Protoplasts
Ion Channels
Arabidopsis
Calcium
Water

ASJC Scopus subject areas

  • General

Cite this

Pel, Z. M., Murata, Y., Benning, G., Thomine, S., Klüsener, B., Allen, G. J., ... Schroeder, J. I. (2000). Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature, 406(6797), 731-734. https://doi.org/10.1038/35021067

Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. / Pel, Zhen Ming; Murata, Yoshiyuki; Benning, Gregor; Thomine, Sébastien; Klüsener, Birgit; Allen, Gethyn J.; Grill, Erwin; Schroeder, Julian I.

In: Nature, Vol. 406, No. 6797, 17.08.2000, p. 731-734.

Research output: Contribution to journalArticle

Pel, ZM, Murata, Y, Benning, G, Thomine, S, Klüsener, B, Allen, GJ, Grill, E & Schroeder, JI 2000, 'Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells', Nature, vol. 406, no. 6797, pp. 731-734. https://doi.org/10.1038/35021067
Pel, Zhen Ming ; Murata, Yoshiyuki ; Benning, Gregor ; Thomine, Sébastien ; Klüsener, Birgit ; Allen, Gethyn J. ; Grill, Erwin ; Schroeder, Julian I. / Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. In: Nature. 2000 ; Vol. 406, No. 6797. pp. 731-734.
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