Calcium channel current in cultured rat mesangial cells

Matomo Nishio, Hirokazu Tsukahara, Masahiro Hiraoka, Masakatsu Sudo, Shigeru Kigoshi, Ikunobu Muramatsu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The presence of voltage-dependent calcium channels has been suggested in mesangial cells by using calcium-sensitive fluorescent probes. However, direct electrophysiological evidence for voltage-dependent calcium channels has not yet been presented. In this study voltage-dependent calcium channels were studied in cultured rat mesangial cells. Whole-cell patch-clamp experiments were done with 50 mM Ba2+ as a charge carrier. Step depolarizing pulses from a holding potential of -50 mV produced an inward barium current at potentials more positive than -10 mV, and a peak current (10-45 pA) was obtained at a membrane potential of approximately +30 mV. The inward current was augmented by 100 nM Bay K 8644, attenuated by 1 μM nifedipine, and abolished by 50 μMCd2+. These results indicate that the inward current is a barium current flowing through L-type calcium channels. This may be the first study that demonstrates the presence of L-type calcium channels in mesangial cells.

Original languageEnglish
Pages (from-to)96-99
Number of pages4
JournalMolecular Pharmacology
Volume43
Issue number1
Publication statusPublished - Jan 1993
Externally publishedYes

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Mesangial Cells
Calcium Channels
L-Type Calcium Channels
Barium
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
Nifedipine
Fluorescent Dyes
Membrane Potentials
Calcium

ASJC Scopus subject areas

  • Pharmacology

Cite this

Nishio, M., Tsukahara, H., Hiraoka, M., Sudo, M., Kigoshi, S., & Muramatsu, I. (1993). Calcium channel current in cultured rat mesangial cells. Molecular Pharmacology, 43(1), 96-99.

Calcium channel current in cultured rat mesangial cells. / Nishio, Matomo; Tsukahara, Hirokazu; Hiraoka, Masahiro; Sudo, Masakatsu; Kigoshi, Shigeru; Muramatsu, Ikunobu.

In: Molecular Pharmacology, Vol. 43, No. 1, 01.1993, p. 96-99.

Research output: Contribution to journalArticle

Nishio, M, Tsukahara, H, Hiraoka, M, Sudo, M, Kigoshi, S & Muramatsu, I 1993, 'Calcium channel current in cultured rat mesangial cells', Molecular Pharmacology, vol. 43, no. 1, pp. 96-99.
Nishio M, Tsukahara H, Hiraoka M, Sudo M, Kigoshi S, Muramatsu I. Calcium channel current in cultured rat mesangial cells. Molecular Pharmacology. 1993 Jan;43(1):96-99.
Nishio, Matomo ; Tsukahara, Hirokazu ; Hiraoka, Masahiro ; Sudo, Masakatsu ; Kigoshi, Shigeru ; Muramatsu, Ikunobu. / Calcium channel current in cultured rat mesangial cells. In: Molecular Pharmacology. 1993 ; Vol. 43, No. 1. pp. 96-99.
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