Calcium channel current in cultured rat mesangial cells

M. Nishio, H. Tsukahara, M. Hiraoka, M. Sudo, S. Kigoshi, I. Muramatsu

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Abstract

The presence of voltage-dependent calcium channels has been suggested in mesangial cells by using calcium-sensitive fluorescent probes. However, direct electrophysiological evidence for voltage-dependent calcium channels has not yet been presented. In this study voltage-dependent calcium channels were studied in cultured rat mesangial cells. Whole-cell patch-clamp experiments were done with 50 mM Ba2+ as a charge carrier. Step depolarizing pulses from a holding potential of -50 mV produced an inward barium current at potentials more positive than -10 mV, and a peak current (10-45 pA) was obtained at a membrane potential of approximately +30 mV. The inward current was augmented by 100 nM Bay K 8644, attenuated by 1 μM nifedipine, and abolished by 50 μM Cd2+. These results indicate that the inward current is a barium current flowing through L-type calcium channels. This may be the first study that demonstrates the presence of L-type calcium channels in mesangial cells.

Original languageEnglish
Pages (from-to)96-99
Number of pages4
JournalMolecular pharmacology
Volume43
Issue number1
Publication statusPublished - Jan 29 1993

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ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Nishio, M., Tsukahara, H., Hiraoka, M., Sudo, M., Kigoshi, S., & Muramatsu, I. (1993). Calcium channel current in cultured rat mesangial cells. Molecular pharmacology, 43(1), 96-99.