Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds

Lamia Sakouhi, Sondès Rahoui, Marouane Ben Massoud, Shintaro Munemasa, Ezzeddine El Ferjani, Yoshiyuki Murata, Abdelilah Chaoui

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Impact of exogenous calcium and ethylene glycol tetraacetic acid (EGTA) supplement on chickpea (Cicer arietinum L.) germinating seeds exposed to cadmium stress for 6 days was studied. Ca and EGTA late treatment (3 days) alleviated growth inhibition and decreased Cd accumulation as well as lipid peroxidation and protein carbonylation in both root and shoot cells. Exogenous effector application relieved Cd-induced cell death which was associated with a constant level of ATP, which was considered as an apoptotic-like process. Redox balance was examined through the study of the redox state of pyridine nucleotide couples NAD+/NADH and NADP+/NADPH as well as their related oxidative [NAD(P)H-oxidase] and dehydrogenase (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malate dehydrogenase) enzyme activities. The present research illustrated an ameliorative effect of Ca and EGTA on growth of Cd-exposed chickpea seedlings that occurs through the protection of sensitive cell sites from Cd-induced oxidation, namely membrane lipids and proteins, rather than the improvement of recycling capabilities of the cellular reducing power.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalJournal of Plant Growth Regulation
DOIs
Publication statusAccepted/In press - May 31 2016

Fingerprint

ethylene glycol tetraacetic acid
cadmium
NAD (coenzyme)
toxicity
calcium
NADP (coenzyme)
seeds
pyridine nucleotides
phosphogluconate dehydrogenase
NAD(P)H dehydrogenase (quinone)
malate dehydrogenase
glucose-6-phosphate 1-dehydrogenase
Cicer arietinum
growth retardation
recycling
cell death
lipid peroxidation
proteins
cells
enzyme activity

Keywords

  • Cadmium
  • Calcium
  • Chickpea
  • EGTA
  • Stress alleviation

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds. / Sakouhi, Lamia; Rahoui, Sondès; Ben Massoud, Marouane; Munemasa, Shintaro; El Ferjani, Ezzeddine; Murata, Yoshiyuki; Chaoui, Abdelilah.

In: Journal of Plant Growth Regulation, 31.05.2016, p. 1-10.

Research output: Contribution to journalArticle

Sakouhi, Lamia ; Rahoui, Sondès ; Ben Massoud, Marouane ; Munemasa, Shintaro ; El Ferjani, Ezzeddine ; Murata, Yoshiyuki ; Chaoui, Abdelilah. / Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds. In: Journal of Plant Growth Regulation. 2016 ; pp. 1-10.
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