Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice

Narumi Hashikawa-Hobara, Takumi Ogawa, Yusuke Sakamoto, Yumi Matsuo, Mami Ogawa, Yoshito Zamami, Naoya Hashikawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) is a neuropeptide that has potent vasodilator properties and is involved in various behavioral disorders. The relationship between CGRP and depression-like behavior is unclear. In this study, we used chronically stressed mice to investigate whether CGRP is involved in depression-like behavior. Each mouse was exposed to restraint and water immersion stress for 15 days. After stress exposure, mice were assessed using behavioral tests: open field test, forced swim test and sucrose preference test. Serum corticosterone levels, hippocampal proliferation and mRNA expression of neurotrophins were measured. After stress exposure, mice exhibited depression-like behavior and decreased CGRP mRNA levels in the hippocampus. Although intracerebroventricular CGRP administration (0.5€‰nmol) did not alter depression-like behavior after 15-day stress exposure, a single CGRP administration into the brain, before the beginning of the 15-day stress exposure, normalized the behavioral dysfunctions and increased nerve growth factor (Ngf) mRNA levels in stressed mice. Furthermore, in the mouse E14 hippocampal cell line, CGRP treatment induced increased expression of Ngf mRNA. The NGF receptor inhibitor K252a inhibited CGRPs antidepressant-like effects in stressed mice. These results suggest that CGRP expression in the mouse hippocampus is associated with depression-like behavior and changes in Ngf mRNA levels.

Original languageEnglish
Article number12559
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - Aug 7 2015

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Calcitonin Gene-Related Peptide
Antidepressive Agents
Depression
Nerve Growth Factor
Messenger RNA
Hippocampus
Nerve Growth Factor Receptor
Nerve Growth Factors
Immersion
Corticosterone
Neuropeptides
Vasodilator Agents
Dehydration
Sucrose
Cell Line
Brain
Serum

ASJC Scopus subject areas

  • General

Cite this

Hashikawa-Hobara, N., Ogawa, T., Sakamoto, Y., Matsuo, Y., Ogawa, M., Zamami, Y., & Hashikawa, N. (2015). Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice. Scientific Reports, 5, [12559]. https://doi.org/10.1038/srep12559

Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice. / Hashikawa-Hobara, Narumi; Ogawa, Takumi; Sakamoto, Yusuke; Matsuo, Yumi; Ogawa, Mami; Zamami, Yoshito; Hashikawa, Naoya.

In: Scientific Reports, Vol. 5, 12559, 07.08.2015.

Research output: Contribution to journalArticle

Hashikawa-Hobara, N, Ogawa, T, Sakamoto, Y, Matsuo, Y, Ogawa, M, Zamami, Y & Hashikawa, N 2015, 'Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice', Scientific Reports, vol. 5, 12559. https://doi.org/10.1038/srep12559
Hashikawa-Hobara N, Ogawa T, Sakamoto Y, Matsuo Y, Ogawa M, Zamami Y et al. Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice. Scientific Reports. 2015 Aug 7;5. 12559. https://doi.org/10.1038/srep12559
Hashikawa-Hobara, Narumi ; Ogawa, Takumi ; Sakamoto, Yusuke ; Matsuo, Yumi ; Ogawa, Mami ; Zamami, Yoshito ; Hashikawa, Naoya. / Calcitonin gene-related peptide pre-administration acts as a novel antidepressant in stressed mice. In: Scientific Reports. 2015 ; Vol. 5.
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