Calcification process dynamics in coral primary polyps as observed using a calcein incubation method

Yoshikazu Ohno, Akira Iguchi, Chuya Shinzato, Mikako Gushi, Mayuri Takeuchi, Atsushi Suzuki, Kazuhiko Sakai, Takashi Nakamura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Calcification processes are largely unknown in scleractinian corals. In this study, live confocal imaging was used to elucidate the spatiotemporal dynamics of the calcification process in aposymbiotic primary polyps of the coral species Acropora digitifera. The fluorophore calcein was used as a calcium deposition marker and a visible indicator of extracellular fluid distribution at the tissue-skeleton interface (subcalicoblastic medium, SCM) in primary polyp tissues. Under continuous incubation in calcein-containing seawater, initial crystallization and skeletal growth were visualized among the calicoblastic cells in live primary polyp tissues. Additionally, the distribution of calcein-stained SCM and contraction movements of the pockets of SCM were captured at intervals of a few minutes. Our experimental system provided several new insights into coral calcification, particularly as a first step in monitoring the relationship between cellular dynamics and calcification in vivo. Our study suggests that coral calcification initiates at intercellular spaces, a finding that may contribute to the general understanding of coral calcification processes.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalBiochemistry and Biophysics Reports
Volume9
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Anthozoa
Polyps
Tissue
Fluorophores
Crystallization
Seawater
Calcium
Extracellular Fluid
Imaging techniques
Extracellular Space
Tissue Distribution
Fluids
Skeleton
Monitoring
fluorexon
Growth

Keywords

  • Calcein
  • Calcification
  • Live imaging
  • Primary aposymbiotic coral polyp
  • Sub calicoblastic medium

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Calcification process dynamics in coral primary polyps as observed using a calcein incubation method. / Ohno, Yoshikazu; Iguchi, Akira; Shinzato, Chuya; Gushi, Mikako; Takeuchi, Mayuri; Suzuki, Atsushi; Sakai, Kazuhiko; Nakamura, Takashi.

In: Biochemistry and Biophysics Reports, Vol. 9, 01.03.2017, p. 289-294.

Research output: Contribution to journalArticle

Ohno, Yoshikazu ; Iguchi, Akira ; Shinzato, Chuya ; Gushi, Mikako ; Takeuchi, Mayuri ; Suzuki, Atsushi ; Sakai, Kazuhiko ; Nakamura, Takashi. / Calcification process dynamics in coral primary polyps as observed using a calcein incubation method. In: Biochemistry and Biophysics Reports. 2017 ; Vol. 9. pp. 289-294.
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