Abstract
The permeation of calcein across the phospholipid bilayer membrane is a key phenomenon in the detection system using liposomes as a sensor unit. The behavior of the calcein release from the liposome was analyzed by a first-order kinetic to obtain the permeability coefficient, Ps [cm/s]. The Ps value for the neutral liposome, prepared by zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was found to depend on both the diameter of liposome and the temperature. The membrane fluidity of the POPC liposome, evaluated by the hydrophobic probe, 1-(4-trimethyl-aminophenyl)-6-diphenyl-1,3,5-hexatriene, was also dependent on the liposome diameter and the temperature. The Ps values for various neutral liposomes under gel phase or liquid-crystalline phase were correlated with their membrane fluidity, although some data were a little scattered, possibly due to the lamellarity. It is therefore considered that the membrane fluidity dominates the permeability of calcein across the neutral phospholipid membrane. Based on the above results, the Ps value for liposomes immobilized on the solid surface is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 156-160 |
| Number of pages | 5 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 73 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 1 2009 |
| Externally published | Yes |
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Keywords
- Calcein permeation
- Immobilization
- Liposome
- Membrane fluidity
- Size
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Calcein permeation across phosphatidylcholine bilayer membrane : Effects of membrane fluidity, liposome size, and immobilization. / Shimanouchi, Toshinori; Ishii, Haruyuki; Yoshimoto, Noriko; Umakoshi, Hiroshi; Kuboi, Ryoichi.
In: Colloids and Surfaces B: Biointerfaces, Vol. 73, No. 1, 01.10.2009, p. 156-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calcein permeation across phosphatidylcholine bilayer membrane
T2 - Effects of membrane fluidity, liposome size, and immobilization
AU - Shimanouchi, Toshinori
AU - Ishii, Haruyuki
AU - Yoshimoto, Noriko
AU - Umakoshi, Hiroshi
AU - Kuboi, Ryoichi
PY - 2009/10/1
Y1 - 2009/10/1
N2 - The permeation of calcein across the phospholipid bilayer membrane is a key phenomenon in the detection system using liposomes as a sensor unit. The behavior of the calcein release from the liposome was analyzed by a first-order kinetic to obtain the permeability coefficient, Ps [cm/s]. The Ps value for the neutral liposome, prepared by zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was found to depend on both the diameter of liposome and the temperature. The membrane fluidity of the POPC liposome, evaluated by the hydrophobic probe, 1-(4-trimethyl-aminophenyl)-6-diphenyl-1,3,5-hexatriene, was also dependent on the liposome diameter and the temperature. The Ps values for various neutral liposomes under gel phase or liquid-crystalline phase were correlated with their membrane fluidity, although some data were a little scattered, possibly due to the lamellarity. It is therefore considered that the membrane fluidity dominates the permeability of calcein across the neutral phospholipid membrane. Based on the above results, the Ps value for liposomes immobilized on the solid surface is discussed.
AB - The permeation of calcein across the phospholipid bilayer membrane is a key phenomenon in the detection system using liposomes as a sensor unit. The behavior of the calcein release from the liposome was analyzed by a first-order kinetic to obtain the permeability coefficient, Ps [cm/s]. The Ps value for the neutral liposome, prepared by zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was found to depend on both the diameter of liposome and the temperature. The membrane fluidity of the POPC liposome, evaluated by the hydrophobic probe, 1-(4-trimethyl-aminophenyl)-6-diphenyl-1,3,5-hexatriene, was also dependent on the liposome diameter and the temperature. The Ps values for various neutral liposomes under gel phase or liquid-crystalline phase were correlated with their membrane fluidity, although some data were a little scattered, possibly due to the lamellarity. It is therefore considered that the membrane fluidity dominates the permeability of calcein across the neutral phospholipid membrane. Based on the above results, the Ps value for liposomes immobilized on the solid surface is discussed.
KW - Calcein permeation
KW - Immobilization
KW - Liposome
KW - Membrane fluidity
KW - Size
UR - http://www.scopus.com/inward/record.url?scp=67650476618&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650476618&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2009.05.014
DO - 10.1016/j.colsurfb.2009.05.014
M3 - Article
VL - 73
SP - 156
EP - 160
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 1
ER -