Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat

Hiroyuki Ichikawa, Toru Deguchi, Yoshiaki Fujiyoshi, Tadao Nakago, David M. Jacobowitz, Tomosada Sugimoto

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Abstract

The cell body size and coexpression of carbonic anhydrase (CA), calretinin (CR) and calcitonin gene-related peptide (CGRP) of primary neurons with calbindin-D28k (CB) was examined in the trigeminal ganglion (TG) of the rat. CB-immunoreactive (-ir) cells were mostly large and preferentially distributed in the maxillary and mandibular divisions of the TG. 48% of CB-ir TG cells exhibited enzyme CA activity. 10% of CB-ir TG cells contained CR-ir. Most TG cells coexpressing CB- and CR-irs were localized to the maxillary and mandibular divisions and exhibited CA activity. 6.5% of CB-ir TG cells coexisted with CGRP-ir. 46% of TG cells coexpressing CB and CGRP exhibited CA activity. The innervation of the molar tooth pulp by CB-ir TG primary neurons was also examined. CB-ir thick and smooth nerve fibers projected from the root pulp to the pulp horn and the roof of the pulp chamber, where they became thinner and rarely entered the subodontoblastic layer. However, they could not be traced to the odontoblastic layer, predentin or dentine. The distribution pattern of CB-ir pulpal fibers was different from that of CR-ir ones. The trigeminal neuronal cells retrogradely labeled with fast blue (FB) from the maxillary molar tooth pulp contained CB- and CR-irs. 23% and 1% of the labeled cells were immunoreactive for CB and CR, respectively. The coexpression of CB- and CR-immunoreactivities (-irs) in FB-labeled cells was negligible. An immunoelectron microscopic method revealed that 21% of pulpal nerve fibers were immunoreactive for CB, and that all CB-ir nerve fibers in the root pulp were myelinated. The present study indicated that the tooth pulp primary neurons contained CB-ir but did not coexpress CB- and CR-irs and that these neurons projected their myelinated axons to the pulp.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalBrain Research
Volume715
Issue number1-2
DOIs
Publication statusPublished - Apr 9 1996

Fingerprint

Calbindin 1
Trigeminal Ganglion
Tooth
Neurons
Calbindin 2
Carbonic Anhydrases
Calcitonin Gene-Related Peptide
Nerve Fibers

Keywords

  • calbindin-D28k
  • calretinin
  • carbonic anhydrase
  • immunohistochemistry
  • rat
  • tooth pulp
  • trigeminal ganglion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ichikawa, H., Deguchi, T., Fujiyoshi, Y., Nakago, T., Jacobowitz, D. M., & Sugimoto, T. (1996). Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. Brain Research, 715(1-2), 71-78. https://doi.org/10.1016/0006-8993(95)01550-7

Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. / Ichikawa, Hiroyuki; Deguchi, Toru; Fujiyoshi, Yoshiaki; Nakago, Tadao; Jacobowitz, David M.; Sugimoto, Tomosada.

In: Brain Research, Vol. 715, No. 1-2, 09.04.1996, p. 71-78.

Research output: Contribution to journalArticle

Ichikawa, H, Deguchi, T, Fujiyoshi, Y, Nakago, T, Jacobowitz, DM & Sugimoto, T 1996, 'Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat', Brain Research, vol. 715, no. 1-2, pp. 71-78. https://doi.org/10.1016/0006-8993(95)01550-7
Ichikawa H, Deguchi T, Fujiyoshi Y, Nakago T, Jacobowitz DM, Sugimoto T. Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. Brain Research. 1996 Apr 9;715(1-2):71-78. https://doi.org/10.1016/0006-8993(95)01550-7
Ichikawa, Hiroyuki ; Deguchi, Toru ; Fujiyoshi, Yoshiaki ; Nakago, Tadao ; Jacobowitz, David M. ; Sugimoto, Tomosada. / Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. In: Brain Research. 1996 ; Vol. 715, No. 1-2. pp. 71-78.
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abstract = "The cell body size and coexpression of carbonic anhydrase (CA), calretinin (CR) and calcitonin gene-related peptide (CGRP) of primary neurons with calbindin-D28k (CB) was examined in the trigeminal ganglion (TG) of the rat. CB-immunoreactive (-ir) cells were mostly large and preferentially distributed in the maxillary and mandibular divisions of the TG. 48{\%} of CB-ir TG cells exhibited enzyme CA activity. 10{\%} of CB-ir TG cells contained CR-ir. Most TG cells coexpressing CB- and CR-irs were localized to the maxillary and mandibular divisions and exhibited CA activity. 6.5{\%} of CB-ir TG cells coexisted with CGRP-ir. 46{\%} of TG cells coexpressing CB and CGRP exhibited CA activity. The innervation of the molar tooth pulp by CB-ir TG primary neurons was also examined. CB-ir thick and smooth nerve fibers projected from the root pulp to the pulp horn and the roof of the pulp chamber, where they became thinner and rarely entered the subodontoblastic layer. However, they could not be traced to the odontoblastic layer, predentin or dentine. The distribution pattern of CB-ir pulpal fibers was different from that of CR-ir ones. The trigeminal neuronal cells retrogradely labeled with fast blue (FB) from the maxillary molar tooth pulp contained CB- and CR-irs. 23{\%} and 1{\%} of the labeled cells were immunoreactive for CB and CR, respectively. The coexpression of CB- and CR-immunoreactivities (-irs) in FB-labeled cells was negligible. An immunoelectron microscopic method revealed that 21{\%} of pulpal nerve fibers were immunoreactive for CB, and that all CB-ir nerve fibers in the root pulp were myelinated. The present study indicated that the tooth pulp primary neurons contained CB-ir but did not coexpress CB- and CR-irs and that these neurons projected their myelinated axons to the pulp.",
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