Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promotong factor activity during nuclear transfer

Manabu Kawahara, Takuya Wakai, Ken Ichi Yamanaka, Jin Kobayashi, Satoshi Sugimura, Takashi Shimizu, Hiromichi Matsumoto, Jin Hoi Kim, Hiroshi Sasada, Eimei Sato

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

When the nucleus in G0/G1 phase is transferred to an enucleated oocyte by nuclear transfer (NT), its nuclear envelope is broken, followed by condensation of chromosome structure by maturation promoting factor (MPF). This morphological remodeling of the transferred interphase nucleus seems to be essential for subsequent development of NT embryos. In this study, we treated porcine NT embryos with caffeine, which has been reported to increase MPF activity, to keep their MPF level high during NT. When 2.5 mM caffeine was added to the handling medium, the proportion of NT embryos showing condensed chromosome increased significantly (P <0.05). In NT embryos treated with caffeine, the activity of p34cdc2 kinase was significantly (P <0.05) higher than in those without caffeine at 3 h post-injection. In addition, the rate of development to the blastocyst stage after activation was significantly (P <0.05) higher in NT embryos treated with caffeine. These results indicate that caffeine treatment can increase not only the rate of chromosome condensation but also the developmental rate to the blastocyst stage of porcine NT embryos. This action is most likely due to the support/increase of MPF activity throughout the process of NT.

Original languageEnglish
Pages (from-to)351-357
Number of pages7
JournalReproduction
Volume130
Issue number3
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

Fingerprint

Embryo Transfer
Caffeine
Maturation-Promoting Factor
Swine
Embryonic Structures
Chromosomes
Blastocyst
Chromosome Structures
Cell Cycle Resting Phase
Interphase
Nuclear Envelope
G1 Phase
Oocytes
In Vitro Techniques
Phosphotransferases
Injections

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Cell Biology
  • Endocrinology
  • Embryology

Cite this

Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promotong factor activity during nuclear transfer. / Kawahara, Manabu; Wakai, Takuya; Yamanaka, Ken Ichi; Kobayashi, Jin; Sugimura, Satoshi; Shimizu, Takashi; Matsumoto, Hiromichi; Kim, Jin Hoi; Sasada, Hiroshi; Sato, Eimei.

In: Reproduction, Vol. 130, No. 3, 09.2005, p. 351-357.

Research output: Contribution to journalArticle

Kawahara, Manabu ; Wakai, Takuya ; Yamanaka, Ken Ichi ; Kobayashi, Jin ; Sugimura, Satoshi ; Shimizu, Takashi ; Matsumoto, Hiromichi ; Kim, Jin Hoi ; Sasada, Hiroshi ; Sato, Eimei. / Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promotong factor activity during nuclear transfer. In: Reproduction. 2005 ; Vol. 130, No. 3. pp. 351-357.
@article{0db09f71f3bf422c8cf4827b5f0dc32a,
title = "Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promotong factor activity during nuclear transfer",
abstract = "When the nucleus in G0/G1 phase is transferred to an enucleated oocyte by nuclear transfer (NT), its nuclear envelope is broken, followed by condensation of chromosome structure by maturation promoting factor (MPF). This morphological remodeling of the transferred interphase nucleus seems to be essential for subsequent development of NT embryos. In this study, we treated porcine NT embryos with caffeine, which has been reported to increase MPF activity, to keep their MPF level high during NT. When 2.5 mM caffeine was added to the handling medium, the proportion of NT embryos showing condensed chromosome increased significantly (P <0.05). In NT embryos treated with caffeine, the activity of p34cdc2 kinase was significantly (P <0.05) higher than in those without caffeine at 3 h post-injection. In addition, the rate of development to the blastocyst stage after activation was significantly (P <0.05) higher in NT embryos treated with caffeine. These results indicate that caffeine treatment can increase not only the rate of chromosome condensation but also the developmental rate to the blastocyst stage of porcine NT embryos. This action is most likely due to the support/increase of MPF activity throughout the process of NT.",
author = "Manabu Kawahara and Takuya Wakai and Yamanaka, {Ken Ichi} and Jin Kobayashi and Satoshi Sugimura and Takashi Shimizu and Hiromichi Matsumoto and Kim, {Jin Hoi} and Hiroshi Sasada and Eimei Sato",
year = "2005",
month = "9",
doi = "10.1530/rep.1.00644",
language = "English",
volume = "130",
pages = "351--357",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd.",
number = "3",

}

TY - JOUR

T1 - Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promotong factor activity during nuclear transfer

AU - Kawahara, Manabu

AU - Wakai, Takuya

AU - Yamanaka, Ken Ichi

AU - Kobayashi, Jin

AU - Sugimura, Satoshi

AU - Shimizu, Takashi

AU - Matsumoto, Hiromichi

AU - Kim, Jin Hoi

AU - Sasada, Hiroshi

AU - Sato, Eimei

PY - 2005/9

Y1 - 2005/9

N2 - When the nucleus in G0/G1 phase is transferred to an enucleated oocyte by nuclear transfer (NT), its nuclear envelope is broken, followed by condensation of chromosome structure by maturation promoting factor (MPF). This morphological remodeling of the transferred interphase nucleus seems to be essential for subsequent development of NT embryos. In this study, we treated porcine NT embryos with caffeine, which has been reported to increase MPF activity, to keep their MPF level high during NT. When 2.5 mM caffeine was added to the handling medium, the proportion of NT embryos showing condensed chromosome increased significantly (P <0.05). In NT embryos treated with caffeine, the activity of p34cdc2 kinase was significantly (P <0.05) higher than in those without caffeine at 3 h post-injection. In addition, the rate of development to the blastocyst stage after activation was significantly (P <0.05) higher in NT embryos treated with caffeine. These results indicate that caffeine treatment can increase not only the rate of chromosome condensation but also the developmental rate to the blastocyst stage of porcine NT embryos. This action is most likely due to the support/increase of MPF activity throughout the process of NT.

AB - When the nucleus in G0/G1 phase is transferred to an enucleated oocyte by nuclear transfer (NT), its nuclear envelope is broken, followed by condensation of chromosome structure by maturation promoting factor (MPF). This morphological remodeling of the transferred interphase nucleus seems to be essential for subsequent development of NT embryos. In this study, we treated porcine NT embryos with caffeine, which has been reported to increase MPF activity, to keep their MPF level high during NT. When 2.5 mM caffeine was added to the handling medium, the proportion of NT embryos showing condensed chromosome increased significantly (P <0.05). In NT embryos treated with caffeine, the activity of p34cdc2 kinase was significantly (P <0.05) higher than in those without caffeine at 3 h post-injection. In addition, the rate of development to the blastocyst stage after activation was significantly (P <0.05) higher in NT embryos treated with caffeine. These results indicate that caffeine treatment can increase not only the rate of chromosome condensation but also the developmental rate to the blastocyst stage of porcine NT embryos. This action is most likely due to the support/increase of MPF activity throughout the process of NT.

UR - http://www.scopus.com/inward/record.url?scp=24944552711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944552711&partnerID=8YFLogxK

U2 - 10.1530/rep.1.00644

DO - 10.1530/rep.1.00644

M3 - Article

C2 - 16123242

AN - SCOPUS:24944552711

VL - 130

SP - 351

EP - 357

JO - Reproduction

JF - Reproduction

SN - 1470-1626

IS - 3

ER -