Caenorhabditis elegans cDNA for a Menkes/Wilson disease gene homologue and its function in a yeast CCC2 gene deletion mutant

Yoshihiro Sambongi, Tokumitsu Wakabayashi, Takao Yoshimizu, Hiroshi Omote, Toshihiko Oka, Masamitsu Futai

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The full-length cDNA coding for a putative copper transporting P-type ATPase (Cu2+-ATPase) was cloned from Caenorhabditis elegans. The putative Cu2+-ATPase is a 1,238-amino acid protein, and highly homologous to the Menkes and Wilson disease gene products mutations of which are responsible for human defects of copper metabolism. The Saccharomyces cerevisiae mutant with a disrupted CCC2 gene (yeast Menkes/Wilson disease gene homologue) was rescued by the cDNA for the C. elegans Cu2+-ATPase but not by the cDNA with an Asp-786 (an invariant phosphorylation site) to Asn mutation, suggesting that the C. elegans Cu2+-ATPase functions as a copper transporter in yeast. The expressed C. elegans protein was detected in yeast vacuolar membranes by immunofluorescence microscopy. The yeast expression system may facilitate further studies on copper transporting P-type ATPases.

Original languageEnglish
Pages (from-to)1169-1175
Number of pages7
JournalJournal of Biochemistry
Volume121
Issue number6
Publication statusPublished - Jun 1997
Externally publishedYes

Fingerprint

Menkes Kinky Hair Syndrome
Hepatolenticular Degeneration
Gene Deletion
Caenorhabditis elegans
Yeast
Adenosine Triphosphatases
Complementary DNA
Genes
Yeasts
Copper
Caenorhabditis elegans Proteins
Mutation
Fluorescence Microscopy
Saccharomyces cerevisiae
Phosphorylation
Amino Acids
Metabolism
Membranes
Microscopic examination
Defects

Keywords

  • Caenorhabditis elegans
  • Copper transporting ATPase
  • Immunofluorescence microscopy
  • Menkes/Wilson disease gene homologue
  • Yeast CCC2 gene

ASJC Scopus subject areas

  • Biochemistry

Cite this

Caenorhabditis elegans cDNA for a Menkes/Wilson disease gene homologue and its function in a yeast CCC2 gene deletion mutant. / Sambongi, Yoshihiro; Wakabayashi, Tokumitsu; Yoshimizu, Takao; Omote, Hiroshi; Oka, Toshihiko; Futai, Masamitsu.

In: Journal of Biochemistry, Vol. 121, No. 6, 06.1997, p. 1169-1175.

Research output: Contribution to journalArticle

Sambongi, Yoshihiro ; Wakabayashi, Tokumitsu ; Yoshimizu, Takao ; Omote, Hiroshi ; Oka, Toshihiko ; Futai, Masamitsu. / Caenorhabditis elegans cDNA for a Menkes/Wilson disease gene homologue and its function in a yeast CCC2 gene deletion mutant. In: Journal of Biochemistry. 1997 ; Vol. 121, No. 6. pp. 1169-1175.
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