Caenorhabditis elegans cDNA for a Menkes/Wilson disease gene homologue and its function in a yeast CCC2 gene deletion mutant

Yoshihiro Sambongi, Tokumitsu Wakabayashi, Takao Yoshimizu, Hiroshi Omote, Toshihiko Oka, Masamitsu Futai

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The full-length cDNA coding for a putative copper transporting P-type ATPase (Cu2+-ATPase) was cloned from Caenorhabditis elegans. The putative Cu2+-ATPase is a 1,238-amino acid protein, and highly homologous to the Menkes and Wilson disease gene products mutations of which are responsible for human defects of copper metabolism. The Saccharomyces cerevisiae mutant with a disrupted CCC2 gene (yeast Menkes/Wilson disease gene homologue) was rescued by the cDNA for the C. elegans Cu2+-ATPase but not by the cDNA with an Asp-786 (an invariant phosphorylation site) to Asn mutation, suggesting that the C. elegans Cu2+-ATPase functions as a copper transporter in yeast. The expressed C. elegans protein was detected in yeast vacuolar membranes by immunofluorescence microscopy. The yeast expression system may facilitate further studies on copper transporting P-type ATPases.

Original languageEnglish
Pages (from-to)1169-1175
Number of pages7
JournalJournal of biochemistry
Volume121
Issue number6
DOIs
Publication statusPublished - Jun 1997
Externally publishedYes

Keywords

  • Caenorhabditis elegans
  • Copper transporting ATPase
  • Immunofluorescence microscopy
  • Menkes/Wilson disease gene homologue
  • Yeast CCC2 gene

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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