Cadmium uptake via apoplastic bypass flow in Oryza sativa

Izumi C. Mori, Carlos Raul Arias-Barreiro, Lia Ooi, Nam Hee Lee, Muhammad Abdus Sobahan, Yoshimasa Nakamura, Yoshihiko Hirai, Yoshiyuki Murata

Research output: Contribution to journalArticlepeer-review

Abstract

It is known that rice roots take up cadmium (Cd) via the symplastic route mediated by membrane-bound mineral transporters. Here we provide evidence that apoplastic bypass flow is another Cd uptake route in rice. High concentrations of Cd rendered apoplastic bypass flow rate increased in rice seedlings. These concentrations of Cd compromised membrane integrity in the root meristem and transition zone. Polyethleneglycol and proline inhibited the Cd-induced apoplastic bypass flow and Cd transfer to the shoots. Loss-of-function mutant of the Cd uptake transporter, nramp5, showed Cd transport to the shoot comparable to the wild type. At a low Cd concentration, increased apoplastic bypass flow rate by NaCl stress resulted in an elevation of Cd transport to shoots both in the wildtype and nramp5. These observations indicate that apoplastic bypass flow in roots carries Cd transport leading to xylem loading of Cd in addition to the symplastic pathway mediated by mineral transporters under stressed conditions.

Original languageEnglish
Pages (from-to)1139-1148
Number of pages10
JournalJournal of Plant Research
Volume134
Issue number5
DOIs
Publication statusPublished - Sep 2021

Keywords

  • Apoplastic bypass flow
  • Cadmium
  • Rice

ASJC Scopus subject areas

  • Plant Science

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