Ca2+ stores in purkinje neurons

Endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca2+-ATPase, and calsequestrin

Kohji Takei, H. Stukenbrok, A. Metcalf, G. A. Mignery, T. C. Südhof, P. Volpe, P. De Camilli

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

The nature of second messenger-responsive intracellular Ca2+ stores in neurons remains open for discussion. Here, we demonstrate the existence in Purkinje cells (PCs) of endoplasmic reticulum (ER) subcompartments characterized by an uneven distribution of three proteins involved in Ca2+ storage and release: the inositol 1,4,5-trisphosphate (InsP3) receptor, Ca2+-ATPase, and calsequestrin. Ca2+-ATPase and the InsP3 receptor have a widespread, although not identical, distribution throughout the ER. Calsequestrin is localized throughout the smooth ER and is particularly concentrated in pleiomorphic vesicles with a moderately electron-dense core, which appear to represent a subcompartment of the smooth ER. In double-labeling experiments many of these vesicles were unlabeled by InsP3 receptor antibodies. These results suggest a key role of the ER as an intracellular Ca2+ store and demonstrate a possible structural basis for distinct intracellular Ca2+ pools regulated by different second messengers.

Original languageEnglish
Pages (from-to)489-505
Number of pages17
JournalJournal of Neuroscience
Volume12
Issue number2
Publication statusPublished - 1992
Externally publishedYes

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Calsequestrin
Calcium-Transporting ATPases
Purkinje Cells
Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
Second Messenger Systems
Inositol 1,4,5-Trisphosphate Receptors
Electrons
Neurons
Antibodies
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

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Ca2+ stores in purkinje neurons : Endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca2+-ATPase, and calsequestrin. / Takei, Kohji; Stukenbrok, H.; Metcalf, A.; Mignery, G. A.; Südhof, T. C.; Volpe, P.; De Camilli, P.

In: Journal of Neuroscience, Vol. 12, No. 2, 1992, p. 489-505.

Research output: Contribution to journalArticle

Takei, Kohji ; Stukenbrok, H. ; Metcalf, A. ; Mignery, G. A. ; Südhof, T. C. ; Volpe, P. ; De Camilli, P. / Ca2+ stores in purkinje neurons : Endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca2+-ATPase, and calsequestrin. In: Journal of Neuroscience. 1992 ; Vol. 12, No. 2. pp. 489-505.
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