Ca2+ regulation of outward rectifying K+ channel in the plasma membrane of tobacco cultured cells in suspension: A role of the K+ channel in mitigation of salt-stress effects by external Ca2+

Yoshiyuki Murata, Manabu Yoshihashi, Ichiro Obi, Tadaaki Kakutani

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The patch-clamp technique was used to study effect of the Ca2+ on K+ channels in the plasma membrane of protoplasts isolated from tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells in suspension. The outward rectifying whole-cell K+ currents were not affected by intracellular Ca2+, but they were reduced with increasing extracellular Ca2+. Neither extracellular nor intracellular Ca2+ affected the permeability ratios (P(K)+/P(Na)+) of the plasma membrane. These results suggest that the inhibition of outward-rectifying K+ channels by extracellular Ca2+ may partially contribute towards the mitigation of detrimental effects of salinity on growth by extracellular Ca2+.

Original languageEnglish
Pages (from-to)1039-1044
Number of pages6
JournalPlant and Cell Physiology
Volume39
Issue number10
Publication statusPublished - Oct 1998

Fingerprint

potassium channels
cultured cells
cell suspension culture
Tobacco
salt stress
Cultured Cells
Suspensions
tobacco
plasma membrane
Salts
Cell Membrane
calcium
Protoplasts
Salinity
Patch-Clamp Techniques
Permeability
Growth
patch-clamp technique
Nicotiana tabacum
protoplasts

Keywords

  • Ca mitigation of salt stress
  • Nicotiana tabacum L.
  • Outward rectifying K channel
  • Patch clamp
  • Plasma membrane

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Physiology

Cite this

@article{002a52a0a52d48e784047ccf5830a0df,
title = "Ca2+ regulation of outward rectifying K+ channel in the plasma membrane of tobacco cultured cells in suspension: A role of the K+ channel in mitigation of salt-stress effects by external Ca2+",
abstract = "The patch-clamp technique was used to study effect of the Ca2+ on K+ channels in the plasma membrane of protoplasts isolated from tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells in suspension. The outward rectifying whole-cell K+ currents were not affected by intracellular Ca2+, but they were reduced with increasing extracellular Ca2+. Neither extracellular nor intracellular Ca2+ affected the permeability ratios (P(K)+/P(Na)+) of the plasma membrane. These results suggest that the inhibition of outward-rectifying K+ channels by extracellular Ca2+ may partially contribute towards the mitigation of detrimental effects of salinity on growth by extracellular Ca2+.",
keywords = "Ca mitigation of salt stress, Nicotiana tabacum L., Outward rectifying K channel, Patch clamp, Plasma membrane",
author = "Yoshiyuki Murata and Manabu Yoshihashi and Ichiro Obi and Tadaaki Kakutani",
year = "1998",
month = "10",
language = "English",
volume = "39",
pages = "1039--1044",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Ca2+ regulation of outward rectifying K+ channel in the plasma membrane of tobacco cultured cells in suspension

T2 - A role of the K+ channel in mitigation of salt-stress effects by external Ca2+

AU - Murata, Yoshiyuki

AU - Yoshihashi, Manabu

AU - Obi, Ichiro

AU - Kakutani, Tadaaki

PY - 1998/10

Y1 - 1998/10

N2 - The patch-clamp technique was used to study effect of the Ca2+ on K+ channels in the plasma membrane of protoplasts isolated from tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells in suspension. The outward rectifying whole-cell K+ currents were not affected by intracellular Ca2+, but they were reduced with increasing extracellular Ca2+. Neither extracellular nor intracellular Ca2+ affected the permeability ratios (P(K)+/P(Na)+) of the plasma membrane. These results suggest that the inhibition of outward-rectifying K+ channels by extracellular Ca2+ may partially contribute towards the mitigation of detrimental effects of salinity on growth by extracellular Ca2+.

AB - The patch-clamp technique was used to study effect of the Ca2+ on K+ channels in the plasma membrane of protoplasts isolated from tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells in suspension. The outward rectifying whole-cell K+ currents were not affected by intracellular Ca2+, but they were reduced with increasing extracellular Ca2+. Neither extracellular nor intracellular Ca2+ affected the permeability ratios (P(K)+/P(Na)+) of the plasma membrane. These results suggest that the inhibition of outward-rectifying K+ channels by extracellular Ca2+ may partially contribute towards the mitigation of detrimental effects of salinity on growth by extracellular Ca2+.

KW - Ca mitigation of salt stress

KW - Nicotiana tabacum L.

KW - Outward rectifying K channel

KW - Patch clamp

KW - Plasma membrane

UR - http://www.scopus.com/inward/record.url?scp=0031789875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031789875&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031789875

VL - 39

SP - 1039

EP - 1044

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 10

ER -