Ca2+ influx-mediated histamine synthesis and IL-6 release in mast cells activated by monomeric IgE

Satoshi Tanaka, Sonoka Mikura, Eri Hashimoto, Yukihiko Sugimoto, Atsushi Ichikawa

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We previously demonstrated that histamine synthesis is drastically induced upon sensitization with an anti-DNP IgE clone, SPE-7, in IL-3-dependent mouse bone marrow derived mast cells (BMMC). We found that Ca2+ mobilization induced by SPE-7 exhibited a similar profile to the capacitative Ca2+ entry evoked by thapsigargin. Potentials for activation of mast cells were found to vary between different IgE clones, and a monovalent hapten, DNP-lysine, suppressed the activation induced by SPE-7. Ca2+ mobilization induced by SPE-7 was suppressed potently by the specific store-operated Ca2+ channel inhibitor, SK&F 96365, but not at all by Ca2+ channel inhibitors with more broad spectrum, La3+ and Gd3+, whereas the Ca2+ mobilization induced by Ag stimulation was suppressed by these inhibitors. Ca2+ mobilization was also induced by SPE-7 in in vitro differentiated mast cells, although the increases in histamine synthesis and IL-6 release were smaller than those in BMMC. These results suggest that Ca2+ influx operated by a distinct mechanism from that in Ag stimulation is essential for increased histamine synthesis and IL-6 release in mast cells.

Original languageEnglish
Pages (from-to)460-468
Number of pages9
JournalEuropean Journal of Immunology
Volume35
Issue number2
DOIs
Publication statusPublished - Feb 2005
Externally publishedYes

Fingerprint

Mast Cells
Immunoglobulin E
Histamine
Interleukin-6
Clone Cells
Bone Marrow
Thapsigargin
Haptens
Interleukin-3
Lysine

Keywords

  • Ca influx
  • Histamine
  • IgE
  • IL-6
  • Mast cell

ASJC Scopus subject areas

  • Immunology

Cite this

Ca2+ influx-mediated histamine synthesis and IL-6 release in mast cells activated by monomeric IgE. / Tanaka, Satoshi; Mikura, Sonoka; Hashimoto, Eri; Sugimoto, Yukihiko; Ichikawa, Atsushi.

In: European Journal of Immunology, Vol. 35, No. 2, 02.2005, p. 460-468.

Research output: Contribution to journalArticle

Tanaka, Satoshi ; Mikura, Sonoka ; Hashimoto, Eri ; Sugimoto, Yukihiko ; Ichikawa, Atsushi. / Ca2+ influx-mediated histamine synthesis and IL-6 release in mast cells activated by monomeric IgE. In: European Journal of Immunology. 2005 ; Vol. 35, No. 2. pp. 460-468.
@article{22d4ce667efc4edd9df8cced201e63ac,
title = "Ca2+ influx-mediated histamine synthesis and IL-6 release in mast cells activated by monomeric IgE",
abstract = "We previously demonstrated that histamine synthesis is drastically induced upon sensitization with an anti-DNP IgE clone, SPE-7, in IL-3-dependent mouse bone marrow derived mast cells (BMMC). We found that Ca2+ mobilization induced by SPE-7 exhibited a similar profile to the capacitative Ca2+ entry evoked by thapsigargin. Potentials for activation of mast cells were found to vary between different IgE clones, and a monovalent hapten, DNP-lysine, suppressed the activation induced by SPE-7. Ca2+ mobilization induced by SPE-7 was suppressed potently by the specific store-operated Ca2+ channel inhibitor, SK&F 96365, but not at all by Ca2+ channel inhibitors with more broad spectrum, La3+ and Gd3+, whereas the Ca2+ mobilization induced by Ag stimulation was suppressed by these inhibitors. Ca2+ mobilization was also induced by SPE-7 in in vitro differentiated mast cells, although the increases in histamine synthesis and IL-6 release were smaller than those in BMMC. These results suggest that Ca2+ influx operated by a distinct mechanism from that in Ag stimulation is essential for increased histamine synthesis and IL-6 release in mast cells.",
keywords = "Ca influx, Histamine, IgE, IL-6, Mast cell",
author = "Satoshi Tanaka and Sonoka Mikura and Eri Hashimoto and Yukihiko Sugimoto and Atsushi Ichikawa",
year = "2005",
month = "2",
doi = "10.1002/eji.200425622",
language = "English",
volume = "35",
pages = "460--468",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "2",

}

TY - JOUR

T1 - Ca2+ influx-mediated histamine synthesis and IL-6 release in mast cells activated by monomeric IgE

AU - Tanaka, Satoshi

AU - Mikura, Sonoka

AU - Hashimoto, Eri

AU - Sugimoto, Yukihiko

AU - Ichikawa, Atsushi

PY - 2005/2

Y1 - 2005/2

N2 - We previously demonstrated that histamine synthesis is drastically induced upon sensitization with an anti-DNP IgE clone, SPE-7, in IL-3-dependent mouse bone marrow derived mast cells (BMMC). We found that Ca2+ mobilization induced by SPE-7 exhibited a similar profile to the capacitative Ca2+ entry evoked by thapsigargin. Potentials for activation of mast cells were found to vary between different IgE clones, and a monovalent hapten, DNP-lysine, suppressed the activation induced by SPE-7. Ca2+ mobilization induced by SPE-7 was suppressed potently by the specific store-operated Ca2+ channel inhibitor, SK&F 96365, but not at all by Ca2+ channel inhibitors with more broad spectrum, La3+ and Gd3+, whereas the Ca2+ mobilization induced by Ag stimulation was suppressed by these inhibitors. Ca2+ mobilization was also induced by SPE-7 in in vitro differentiated mast cells, although the increases in histamine synthesis and IL-6 release were smaller than those in BMMC. These results suggest that Ca2+ influx operated by a distinct mechanism from that in Ag stimulation is essential for increased histamine synthesis and IL-6 release in mast cells.

AB - We previously demonstrated that histamine synthesis is drastically induced upon sensitization with an anti-DNP IgE clone, SPE-7, in IL-3-dependent mouse bone marrow derived mast cells (BMMC). We found that Ca2+ mobilization induced by SPE-7 exhibited a similar profile to the capacitative Ca2+ entry evoked by thapsigargin. Potentials for activation of mast cells were found to vary between different IgE clones, and a monovalent hapten, DNP-lysine, suppressed the activation induced by SPE-7. Ca2+ mobilization induced by SPE-7 was suppressed potently by the specific store-operated Ca2+ channel inhibitor, SK&F 96365, but not at all by Ca2+ channel inhibitors with more broad spectrum, La3+ and Gd3+, whereas the Ca2+ mobilization induced by Ag stimulation was suppressed by these inhibitors. Ca2+ mobilization was also induced by SPE-7 in in vitro differentiated mast cells, although the increases in histamine synthesis and IL-6 release were smaller than those in BMMC. These results suggest that Ca2+ influx operated by a distinct mechanism from that in Ag stimulation is essential for increased histamine synthesis and IL-6 release in mast cells.

KW - Ca influx

KW - Histamine

KW - IgE

KW - IL-6

KW - Mast cell

UR - http://www.scopus.com/inward/record.url?scp=14744296202&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14744296202&partnerID=8YFLogxK

U2 - 10.1002/eji.200425622

DO - 10.1002/eji.200425622

M3 - Article

C2 - 15668925

AN - SCOPUS:14744296202

VL - 35

SP - 460

EP - 468

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -