Abstract
We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the differentiation induced by shifting up to the non-permissive temperature, a rapid and transient decrease in both c-myc and p53 mRNA levels and rapid induction of c-jun mRNA were observed. These changes were specific in differentiation-inducible mutants and were not observed in a non-inducible mutant. In both types of mutants, the level of c-myc mRNA decreased in association with growth retardation at the non-permissive temperature. The p53 mRNA, however, showed specific increase in the differentiation-inducible ts mutants. These observations suggest distinct roles for p53 and c-myc from proliferation to differentiation in teratocarcinoma stem cells.
| Original language | English |
|---|---|
| Pages (from-to) | 2455-2460 |
| Number of pages | 6 |
| Journal | Oncogene |
| Volume | 7 |
| Issue number | 12 |
| Publication status | Published - Dec 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Cancer Research
- Genetics
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c-myc and p53 gene expression in the differentiation of temperature-sensitive mutants of teratocarcinoma F9 cells. / Kosaka, Mitsuko; Iwai, Souichi A.; Nishina, Yukio; Sumi, Tetsuro; Nishimune, Yoshitake.
In: Oncogene, Vol. 7, No. 12, 12.1992, p. 2455-2460.Research output: Contribution to journal › Article
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TY - JOUR
T1 - c-myc and p53 gene expression in the differentiation of temperature-sensitive mutants of teratocarcinoma F9 cells
AU - Kosaka, Mitsuko
AU - Iwai, Souichi A.
AU - Nishina, Yukio
AU - Sumi, Tetsuro
AU - Nishimune, Yoshitake
PY - 1992/12
Y1 - 1992/12
N2 - We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the differentiation induced by shifting up to the non-permissive temperature, a rapid and transient decrease in both c-myc and p53 mRNA levels and rapid induction of c-jun mRNA were observed. These changes were specific in differentiation-inducible mutants and were not observed in a non-inducible mutant. In both types of mutants, the level of c-myc mRNA decreased in association with growth retardation at the non-permissive temperature. The p53 mRNA, however, showed specific increase in the differentiation-inducible ts mutants. These observations suggest distinct roles for p53 and c-myc from proliferation to differentiation in teratocarcinoma stem cells.
AB - We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the differentiation induced by shifting up to the non-permissive temperature, a rapid and transient decrease in both c-myc and p53 mRNA levels and rapid induction of c-jun mRNA were observed. These changes were specific in differentiation-inducible mutants and were not observed in a non-inducible mutant. In both types of mutants, the level of c-myc mRNA decreased in association with growth retardation at the non-permissive temperature. The p53 mRNA, however, showed specific increase in the differentiation-inducible ts mutants. These observations suggest distinct roles for p53 and c-myc from proliferation to differentiation in teratocarcinoma stem cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026495353&partnerID=8YFLogxK
M3 - Article
C2 - 1461650
AN - SCOPUS:0026495353
VL - 7
SP - 2455
EP - 2460
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 12
ER -