c-myc and p53 gene expression in the differentiation of temperature-sensitive mutants of teratocarcinoma F9 cells

Mitsuko Kosaka, Souichi A. Iwai, Yukio Nishina, Tetsuro Sumi, Yoshitake Nishimune

Research output: Contribution to journalArticle

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Abstract

We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the differentiation induced by shifting up to the non-permissive temperature, a rapid and transient decrease in both c-myc and p53 mRNA levels and rapid induction of c-jun mRNA were observed. These changes were specific in differentiation-inducible mutants and were not observed in a non-inducible mutant. In both types of mutants, the level of c-myc mRNA decreased in association with growth retardation at the non-permissive temperature. The p53 mRNA, however, showed specific increase in the differentiation-inducible ts mutants. These observations suggest distinct roles for p53 and c-myc from proliferation to differentiation in teratocarcinoma stem cells.

Original languageEnglish
Pages (from-to)2455-2460
Number of pages6
JournalOncogene
Volume7
Issue number12
Publication statusPublished - Dec 1992
Externally publishedYes

Fingerprint

Teratocarcinoma
myc Genes
p53 Genes
Gene Expression
Temperature
Messenger RNA
Embryonal Carcinoma Stem Cells
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

c-myc and p53 gene expression in the differentiation of temperature-sensitive mutants of teratocarcinoma F9 cells. / Kosaka, Mitsuko; Iwai, Souichi A.; Nishina, Yukio; Sumi, Tetsuro; Nishimune, Yoshitake.

In: Oncogene, Vol. 7, No. 12, 12.1992, p. 2455-2460.

Research output: Contribution to journalArticle

Kosaka, M, Iwai, SA, Nishina, Y, Sumi, T & Nishimune, Y 1992, 'c-myc and p53 gene expression in the differentiation of temperature-sensitive mutants of teratocarcinoma F9 cells', Oncogene, vol. 7, no. 12, pp. 2455-2460.
Kosaka, Mitsuko ; Iwai, Souichi A. ; Nishina, Yukio ; Sumi, Tetsuro ; Nishimune, Yoshitake. / c-myc and p53 gene expression in the differentiation of temperature-sensitive mutants of teratocarcinoma F9 cells. In: Oncogene. 1992 ; Vol. 7, No. 12. pp. 2455-2460.
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