Abstract
We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the differentiation induced by shifting up to the non-permissive temperature, a rapid and transient decrease in both c-myc and p53 mRNA levels and rapid induction of c-jun mRNA were observed. These changes were specific in differentiation-inducible mutants and were not observed in a non-inducible mutant. In both types of mutants, the level of c-myc mRNA decreased in association with growth retardation at the non-permissive temperature. The p53 mRNA, however, showed specific increase in the differentiation-inducible ts mutants. These observations suggest distinct roles for p53 and c-myc from proliferation to differentiation in teratocarcinoma stem cells.
| Original language | English |
|---|---|
| Pages (from-to) | 2455-2460 |
| Number of pages | 6 |
| Journal | Oncogene |
| Volume | 7 |
| Issue number | 12 |
| Publication status | Published - Dec 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Cancer Research
- Genetics