Budding of fowlpox and pigeonpox viruses at the surface of infected cells

Y. Hatano, M. Yoshida, F. Uno, S. Yoshida, N. Osafune, K. Ono, Masao Yamada

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chick embryo fibroblasts and chorioallantoic membranes infected with fowlpox virus (FWPV) or pigeonpox virus (PPV) were examined by transmission and scanning electron microscopy. Immature virus particles were observed in finely granular areas, i.e. virus factories, of the cytoplasm. These particles had various forms depending on their stages of development. Many tubular structures were also seen in these regions. Mature virus particles with ellipsoidal or brick-shaped forms enclosing electron-dense cores were detected throughout the cytoplasm. Notably, there was a high frequency of virus budding at the cell surface, but only occasional virus wrapping in the cytoplasm. Another remarkable feature of the infected cells was accumulation of many virions just beneath the plasma membrane, indicating that this phenomenon is closely related to virus budding. Based on the observed frequency of budding, this mechanism seems to be the predominant way for FWPV and PPV to exit the cell.

Original languageEnglish
Pages (from-to)113-124
Number of pages12
JournalJournal of Electron Microscopy
Volume50
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Avipoxvirus
Fowlpox virus
viruses
Viruses
Virion
Virus Release
Cytoplasm
cells
Chorioallantoic Membrane
Scanning Transmission Electron Microscopy
Chick Embryo
cytoplasm
Fibroblasts
Cell Membrane
Electrons
membranes
bricks
embryos
fibroblasts
Cell membranes

Keywords

  • Budding
  • Fowlpox
  • Pigeonpox
  • Poxvirus
  • SEM
  • TEM

ASJC Scopus subject areas

  • Instrumentation

Cite this

Budding of fowlpox and pigeonpox viruses at the surface of infected cells. / Hatano, Y.; Yoshida, M.; Uno, F.; Yoshida, S.; Osafune, N.; Ono, K.; Yamada, Masao.

In: Journal of Electron Microscopy, Vol. 50, No. 2, 2001, p. 113-124.

Research output: Contribution to journalArticle

Hatano, Y, Yoshida, M, Uno, F, Yoshida, S, Osafune, N, Ono, K & Yamada, M 2001, 'Budding of fowlpox and pigeonpox viruses at the surface of infected cells', Journal of Electron Microscopy, vol. 50, no. 2, pp. 113-124. https://doi.org/10.1093/jmicro/50.2.113
Hatano, Y. ; Yoshida, M. ; Uno, F. ; Yoshida, S. ; Osafune, N. ; Ono, K. ; Yamada, Masao. / Budding of fowlpox and pigeonpox viruses at the surface of infected cells. In: Journal of Electron Microscopy. 2001 ; Vol. 50, No. 2. pp. 113-124.
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