TY - JOUR
T1 - Brn-3a is required for the generation of proprioceptors in the mesencephalic trigeminal tract nucleus
AU - Ichikawa, H.
AU - Qiu, F.
AU - Xiang, M.
AU - Sugimoto, T.
N1 - Funding Information:
This work was supported in part by a grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology to H.I. (No. 14571733) and by a grant from the National Institutes of Health to M.X. (DC04594).
PY - 2005/8/16
Y1 - 2005/8/16
N2 - The distribution of motor and proprioceptive neurons was investigated in the trigeminal nervous system of wild-type and Brn-3a knockout mice at embryonic day 18.5 and postnatal day 0. We found that the trigeminal motor nucleus (Mo5) contained abundant motoneurons in wild-type (mean number ± SD per section = 128 ± 22, range = 93-167) and knockout (mean number ± SD per section = 121 ± 23, range = 75-158) mice and that the cell size of Mo5 neurons was similar between these mice (wild-type, mean ± SD = 165 ± 59 μm2, range = 65-326 μm2; knockout, mean ± SD = 167 ± 59 μm2, range = 71-327 μm 2). Mo5 neurons were immunoreactive for calcitonin gene-related peptide and such immunoreactive neurons were abundant in both wild-type and mutant mice. In the mesencephalic tract nucleus (Mes5) of wild-type mice, many proprioceptors (mean number ± SD per section = 56 ± 19, range = 27-85) that contained parvalbumin immunoreactivity were also observed. In knockout mice, however, Mes5 neurons could not be detected. The area of brainstems which normally contained the Mes5 was devoid of parvalbumin- immunoreactive proprioceptors. The present study suggests that Brn-3a is required for the development of proprioceptors but not motoneurons in the trigeminal nervous system.
AB - The distribution of motor and proprioceptive neurons was investigated in the trigeminal nervous system of wild-type and Brn-3a knockout mice at embryonic day 18.5 and postnatal day 0. We found that the trigeminal motor nucleus (Mo5) contained abundant motoneurons in wild-type (mean number ± SD per section = 128 ± 22, range = 93-167) and knockout (mean number ± SD per section = 121 ± 23, range = 75-158) mice and that the cell size of Mo5 neurons was similar between these mice (wild-type, mean ± SD = 165 ± 59 μm2, range = 65-326 μm2; knockout, mean ± SD = 167 ± 59 μm2, range = 71-327 μm 2). Mo5 neurons were immunoreactive for calcitonin gene-related peptide and such immunoreactive neurons were abundant in both wild-type and mutant mice. In the mesencephalic tract nucleus (Mes5) of wild-type mice, many proprioceptors (mean number ± SD per section = 56 ± 19, range = 27-85) that contained parvalbumin immunoreactivity were also observed. In knockout mice, however, Mes5 neurons could not be detected. The area of brainstems which normally contained the Mes5 was devoid of parvalbumin- immunoreactive proprioceptors. The present study suggests that Brn-3a is required for the development of proprioceptors but not motoneurons in the trigeminal nervous system.
KW - Immunohistochemistry
KW - Knockout mouse
KW - Parvalbumin
KW - Proprioceptor
KW - Transcription factor
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U2 - 10.1016/j.brainres.2005.06.026
DO - 10.1016/j.brainres.2005.06.026
M3 - Article
C2 - 16040009
AN - SCOPUS:23644462082
SN - 0006-8993
VL - 1053
SP - 203
EP - 206
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -