TY - JOUR
T1 - Brn-3a deficiency increases tyrosine hydroxylase-immunoreactive neurons in the dorsal root ganglion
AU - Ichikawa, Hiroyuki
AU - Mo, Zeqian
AU - Xiang, Mengqing
AU - Sugimoto, Tomosada
N1 - Funding Information:
This work was supported in part by a grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology to H.I. (No. 14571733) and by a grant from the National Institutes of Health to M.X. (DC04594).
PY - 2005/3/2
Y1 - 2005/3/2
N2 - Immunohistochemistry for tyrosine hydroxylase (TH) was performed on the dorsal root ganglia (DRG) in wild-type, heterozygous and Brn-3a knockout mice at embryonic day 18.5. TH-immunoreactive (-IR) neurons were detected in the DRG of wild-type and heterozygous mice, but their proportion was greatly increased by the loss of Brn-3a function (wild-type and heterozygot, 8.4%; knockout, 20.9%). IR neurons were of various sizes in wild-type (mean ± S.D. = 118.1 ± 55.4 μm2, range = 26.6-306.3 μm2) and heterozygous mice. In the knockout mice, however, TH-IR neurons were mostly small (mean ± S.D. = 68.2 ± 34.3 μm2, range = 11.8-166.8 μm2). The present study suggests that Brn-3a may normally suppress TH expression in many small DRG neurons but activate TH expression in large DRG neurons.
AB - Immunohistochemistry for tyrosine hydroxylase (TH) was performed on the dorsal root ganglia (DRG) in wild-type, heterozygous and Brn-3a knockout mice at embryonic day 18.5. TH-immunoreactive (-IR) neurons were detected in the DRG of wild-type and heterozygous mice, but their proportion was greatly increased by the loss of Brn-3a function (wild-type and heterozygot, 8.4%; knockout, 20.9%). IR neurons were of various sizes in wild-type (mean ± S.D. = 118.1 ± 55.4 μm2, range = 26.6-306.3 μm2) and heterozygous mice. In the knockout mice, however, TH-IR neurons were mostly small (mean ± S.D. = 68.2 ± 34.3 μm2, range = 11.8-166.8 μm2). The present study suggests that Brn-3a may normally suppress TH expression in many small DRG neurons but activate TH expression in large DRG neurons.
KW - Brn-3a
KW - Calcitonin gene-related peptide
KW - Dorsal root ganglion
KW - Immunohistochemistry
KW - Knockout mouse
KW - Tyrosine hydroxylase
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U2 - 10.1016/j.brainres.2004.10.028
DO - 10.1016/j.brainres.2004.10.028
M3 - Article
C2 - 15725417
AN - SCOPUS:13844298842
VL - 1036
SP - 192
EP - 195
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -