Brn-3a deficiency increases tyrosine hydroxylase-immunoreactive neurons in the dorsal root ganglion

Hiroyuki Ichikawa; Zeqian Mo; Mengqing Xiang; Tomosada Sugimoto

doi:10.1016/j.brainres.2004.10.028

Brn-3a deficiency increases tyrosine hydroxylase-immunoreactive neurons in the dorsal root ganglion

Hiroyuki Ichikawa, Zeqian Mo, Mengqing Xiang, Tomosada Sugimoto

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Immunohistochemistry for tyrosine hydroxylase (TH) was performed on the dorsal root ganglia (DRG) in wild-type, heterozygous and Brn-3a knockout mice at embryonic day 18.5. TH-immunoreactive (-IR) neurons were detected in the DRG of wild-type and heterozygous mice, but their proportion was greatly increased by the loss of Brn-3a function (wild-type and heterozygot, 8.4%; knockout, 20.9%). IR neurons were of various sizes in wild-type (mean ± S.D. = 118.1 ± 55.4 μm², range = 26.6-306.3 μm²) and heterozygous mice. In the knockout mice, however, TH-IR neurons were mostly small (mean ± S.D. = 68.2 ± 34.3 μm², range = 11.8-166.8 μm²). The present study suggests that Brn-3a may normally suppress TH expression in many small DRG neurons but activate TH expression in large DRG neurons.

Original language	English
Pages (from-to)	192-195
Number of pages	4
Journal	Brain Research
Volume	1036
Issue number	1-2
DOIs	https://doi.org/10.1016/j.brainres.2004.10.028
Publication status	Published - Mar 2 2005

Keywords

Brn-3a
Calcitonin gene-related peptide
Dorsal root ganglion
Immunohistochemistry
Knockout mouse
Tyrosine hydroxylase

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Brn-3a deficiency increases tyrosine hydroxylase-immunoreactive neurons in the dorsal root ganglion",

abstract = "Immunohistochemistry for tyrosine hydroxylase (TH) was performed on the dorsal root ganglia (DRG) in wild-type, heterozygous and Brn-3a knockout mice at embryonic day 18.5. TH-immunoreactive (-IR) neurons were detected in the DRG of wild-type and heterozygous mice, but their proportion was greatly increased by the loss of Brn-3a function (wild-type and heterozygot, 8.4%; knockout, 20.9%). IR neurons were of various sizes in wild-type (mean ± S.D. = 118.1 ± 55.4 μm2, range = 26.6-306.3 μm2) and heterozygous mice. In the knockout mice, however, TH-IR neurons were mostly small (mean ± S.D. = 68.2 ± 34.3 μm2, range = 11.8-166.8 μm2). The present study suggests that Brn-3a may normally suppress TH expression in many small DRG neurons but activate TH expression in large DRG neurons.",

keywords = "Brn-3a, Calcitonin gene-related peptide, Dorsal root ganglion, Immunohistochemistry, Knockout mouse, Tyrosine hydroxylase",

author = "Hiroyuki Ichikawa and Zeqian Mo and Mengqing Xiang and Tomosada Sugimoto",

year = "2005",

month = mar,

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doi = "10.1016/j.brainres.2004.10.028",

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T1 - Brn-3a deficiency increases tyrosine hydroxylase-immunoreactive neurons in the dorsal root ganglion

AU - Ichikawa, Hiroyuki

AU - Mo, Zeqian

AU - Xiang, Mengqing

AU - Sugimoto, Tomosada

PY - 2005/3/2

Y1 - 2005/3/2

N2 - Immunohistochemistry for tyrosine hydroxylase (TH) was performed on the dorsal root ganglia (DRG) in wild-type, heterozygous and Brn-3a knockout mice at embryonic day 18.5. TH-immunoreactive (-IR) neurons were detected in the DRG of wild-type and heterozygous mice, but their proportion was greatly increased by the loss of Brn-3a function (wild-type and heterozygot, 8.4%; knockout, 20.9%). IR neurons were of various sizes in wild-type (mean ± S.D. = 118.1 ± 55.4 μm2, range = 26.6-306.3 μm2) and heterozygous mice. In the knockout mice, however, TH-IR neurons were mostly small (mean ± S.D. = 68.2 ± 34.3 μm2, range = 11.8-166.8 μm2). The present study suggests that Brn-3a may normally suppress TH expression in many small DRG neurons but activate TH expression in large DRG neurons.

AB - Immunohistochemistry for tyrosine hydroxylase (TH) was performed on the dorsal root ganglia (DRG) in wild-type, heterozygous and Brn-3a knockout mice at embryonic day 18.5. TH-immunoreactive (-IR) neurons were detected in the DRG of wild-type and heterozygous mice, but their proportion was greatly increased by the loss of Brn-3a function (wild-type and heterozygot, 8.4%; knockout, 20.9%). IR neurons were of various sizes in wild-type (mean ± S.D. = 118.1 ± 55.4 μm2, range = 26.6-306.3 μm2) and heterozygous mice. In the knockout mice, however, TH-IR neurons were mostly small (mean ± S.D. = 68.2 ± 34.3 μm2, range = 11.8-166.8 μm2). The present study suggests that Brn-3a may normally suppress TH expression in many small DRG neurons but activate TH expression in large DRG neurons.

KW - Brn-3a

KW - Calcitonin gene-related peptide

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KW - Immunohistochemistry

KW - Knockout mouse

KW - Tyrosine hydroxylase

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