TY - JOUR
T1 - Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS
AU - Bekku, Yoko
AU - Rauch, Uwe
AU - Ninomiya, Yoshifumi
AU - Oohashi, Toshitaka
PY - 2009/3
Y1 - 2009/3
N2 - Brevican is known to be an abundant extracellular matrix component in the adult brain and a structural constituent of perineuronal nets. We herein show that brevican, tenascin-R (TN-R) and phosphacan are present at the nodes of Ranvier on myelinated axons with a particularly large diameter in the central nervous system. A brevican deficiency resulted in a reorganization of the nodal matrices, which was characterized by the shift of TN-R, and concomitantly phosphacan, from an axonal diameter-dependent association with nodes to an axonal diameter independent association. Supported by the co-immunoprecipitation results, these observations indicate that the presence of TN-R and phosphacan at nodes is normally brevican-dependent, while in the absence of brevican these molecules can also be recruited by versican V2. The versican V2 and Bral1 distribution was not affected, thus indicating a brevican-independent role of these two molecules for establishing hyaluronan-binding matrices at the nodes. Our results revealed that brevican plays a crucial role in determining the specialization of the hyaluronan-binding nodal matrix assemblies in large diameter nodes.
AB - Brevican is known to be an abundant extracellular matrix component in the adult brain and a structural constituent of perineuronal nets. We herein show that brevican, tenascin-R (TN-R) and phosphacan are present at the nodes of Ranvier on myelinated axons with a particularly large diameter in the central nervous system. A brevican deficiency resulted in a reorganization of the nodal matrices, which was characterized by the shift of TN-R, and concomitantly phosphacan, from an axonal diameter-dependent association with nodes to an axonal diameter independent association. Supported by the co-immunoprecipitation results, these observations indicate that the presence of TN-R and phosphacan at nodes is normally brevican-dependent, while in the absence of brevican these molecules can also be recruited by versican V2. The versican V2 and Bral1 distribution was not affected, thus indicating a brevican-independent role of these two molecules for establishing hyaluronan-binding matrices at the nodes. Our results revealed that brevican plays a crucial role in determining the specialization of the hyaluronan-binding nodal matrix assemblies in large diameter nodes.
KW - Action potential
KW - Extracellular matrix
KW - Node of Ranvier
KW - Proteoglycan
KW - Scaffolding proteins
KW - Tenascin-R
UR - http://www.scopus.com/inward/record.url?scp=59449103926&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59449103926&partnerID=8YFLogxK
U2 - 10.1111/j.1471-4159.2009.05873.x
DO - 10.1111/j.1471-4159.2009.05873.x
M3 - Article
C2 - 19141078
AN - SCOPUS:59449103926
VL - 108
SP - 1266
EP - 1276
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 5
ER -